Identification of the Arabidopsis thaliana flavonoid 3′-hydroxylase gene and functional expression of the encoded P450 enzyme
Identification of the Arabidopsis thaliana flavonoid 3′-hydroxylase gene and functional expression of the encoded P450 enzyme
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DOI：
10.1515/bc.2000.095
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发表时间：
2000-08-01
影响因子：
3.7
通讯作者：
Weisshaar, B
中科院分区：
文献类型：
--
作者：
Schoenbohm, C;Martens, S;Weisshaar, B
苯丙素途径导致数千种化合物的合成，包括黄酮类化合物，如黄酮醇，花青素和单宁。在拟南芥中，种皮（种皮）中缺乏单宁导致透明种皮（tt）表型。在本研究中，我们确定了负责tt 7突变的基因。我们发现TT 7编码类黄酮3 '-羟化酶（F3' H），并证明这种P450依赖性单加氧酶具有F3 'H活性。AtF 3 'H基因和启动子序列的获得将使我们能够研究一套完整的黄酮醇和花青素生物合成基因的共调控。thaliana，使羟基黄酮的体外合成更加可行。
The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3'-hydroxylase (F3'H), and demonstrate that this P450-dependent monooxygenase has F3'H activity. The availability of the AtF3'H gene and promoter sequence will allow us to study the coregulation of a complete set of flavonol and anthocyanidin biosynthesis genes in A. thaliana, and makes in vitro synthesis of hydroxylated flavonoids more feasible.