Pesticides report .33. Immunoassays for residue analysis of agrochemicals: Proposed guidelines for precision, standardization and quality control
Pesticides report .33. Immunoassays for residue analysis of agrochemicals: Proposed guidelines for precision, standardization and quality control
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DOI：
10.1351/pac199567122065
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发表时间：
1995-12-01
影响因子：
1.8
通讯作者：
Zeeh, B
中科院分区：
文献类型：
--
作者：
Krotzky, AJ;Zeeh, B
农用化学品的免疫测定（ELISA）是一种分析技术，它利用抗体作为“生物化学检测器”来定量活性成分或其衍生物。与临床化学相比，免疫分析在农业化学品残留分析中的应用以及注册机构对ELISA数据的接受仍处于起步阶段。过去缺少ELISA的性能质量控制标准，这引起了人们对该技术用于农业化学品分析的可靠性的质疑。本报告提供了免疫分析的基本技术信息，如测试格式和检测系统。这种方法的可靠性，如基质干扰和交叉反应的关键参数进行了讨论。指南提出了精确度要求，标准化的评价程序和农业化学品免疫分析方法的分布。后续活动进行了总结，目的是产生一个广泛的数据基础上的精确性和适用性的免疫测定农业化学分析。
Immunoassays (ELISA) for agrochemicals are analytical techniques which make use of antibodies as ''biochemical detectors'' for the quantification of active ingredients or their derivatives. In contrast to clinical chemistry the application of immunoanalytics to residue analyses of agrochemicals by industries and the acceptance of ELISA data by registration agencies is still in its infancy. Missing performance quality control criteria for ELISA in the past have raised questions about the reliability of this technique for the analysis of agrochemicals. This report gives basic technical information on immunoassays such as test formats and detection systems. Parameters critical for the reliability of this methodology such as matrix interference and cross reactivity are discussed. Guidelines are proposed for precision requirements, standardised evaluation procedures and the distribution of immunoanalytical methods for agrochemicals. Follow-up activities are summarised with the intention of generating a wide data base on the precision and applicability of immunoassays for agrochemical analysis.