Plasmonic polydopamine-modified TiO2 nanotube substrates for surface-assisted laser desorption/ionization mass spectrometry imaging
Plasmonic polydopamine-modified TiO2 nanotube substrates for surface-assisted laser desorption/ionization mass spectrometry imaging
复制标题
用于表面辅助激光解吸/电离质谱成像的等离子体聚多巴胺修饰的 TiO2 纳米管基底
DOI：
10.1007/s12274-022-4924-z
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发表时间：
2022-08-26
期刊：
影响因子：
9.9
通讯作者：
Yin，Zhibin
中科院分区：
文献类型：
--
作者：
Chen，Dong;Du，Mingyi;Yin，Zhibin
质谱成像（MSI）使生物组织内广泛的小分子代谢物的空间化学表征成为可能。然而，现有的基质辅助激光解吸/电离质谱（MALDI-MS）遭受严重的背景干扰，在低质量范围和不均匀的基质沉积。因此，表面辅助LDI-MS（SALDI-MS）已成为高灵敏度检测和小生物分子成像的有吸引力的替代方案。在这项研究中，我们构建了一种新的复合基板，疏水聚多巴胺（hPDA）修饰的二氧化钛纳米管（TDNT）涂覆等离子体金纳米粒子（AuNP-hPDA-TDNT），作为一个双极性SALDI基板使用一个简单和成本效益的制造方法。得益于TDNT半导体和等离子体PDA修饰的协同效应，该SALDI基底表现出上级性能，可用于多种多样的小分子的双极性检测。使用AuNP-hPDA-TDNT底物可以实现高度降低的背景干扰、较低的检测限和点到点的重复性。由于其独特的印迹性能，各种代谢物和脂质可以在小桐子全缘花瓣，银杏叶，草莓果实和潜在指纹中可视化。更有价值的是，这种无基质的普遍性被证明用于绘制小鼠脑组织切片内脂质的空间分布。综合考虑，这种AuNP-hPDA-TDNT材料有望在各个领域，特别是在纳米材料开发和生命科学中成为一种有前途的SALDI基底。
Mass spectrometry imaging (MSI) has made the spatio-chemical characterization of a broad range of small-molecule metabolites within biological tissues possible. However, available matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) suffers from severe background interferences in low-mass ranges and inhomogeneous matrix deposition. Thus, surface-assisted LDI-MS (SALDI-MS) has been an attractive alternative for high-sensitivity detection and imaging of small biomolecules. In this study, we construct a new composite substrate, hydrophobic polydopamine (hPDA)-modified TiO2nanotube (TDNT) coated with plasmonic gold nanoparticle (AuNP-hPDA-TDNT), as a dual-polarity SALDI substrate using an easy and cost-effective fabrication approach. Benefitting from the synergistic effects of TDNT semiconductor and plasmonic PDA modification, this SALDI substrate exhibits superior performance for dual-polarity detection of a vast diversity of small molecules. Highly reduced background interferences, lower detection limits, and spot-to-spot repeatability can be achieved using AuNP-hPDA-TDNT substrates. Due to its unique imprinting performance, various metabolites and lipids can be visualized withinjatropha integerrimapetals, ginkgo leaves, strawberry fruits, and latent fingerprints. More valuably, the universality of this matrix-free substrate is demonstrated for mapping spatial distribution of lipids within mouse brain tissue sections. Considered together, this AuNP-hPDA-TDNT material is expected to be a promising SALDI substrate in various fields, especially in nanomaterial development and life sciences.