GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map
GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map
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GALAXY ver3：更新了基于 3D HPLC 图谱的糖基化分析 Web 应用程序
DOI：
10.1093/glycob/cwac025
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发表时间：
2022
期刊：
影响因子：
4.3
通讯作者：
Kato Koichi
中科院分区：
文献类型：
--
作者：
Yagi Hirokazu;Amagasa Eijiro;Shiota Masaaki;Yamada Issaku;Aoki-Kinoshita Kiyoko F;Kato Koichi
高效液相色谱 (HPLC) 洗脱数据为定量糖基化分析、区分异构寡糖提供了有用的工具。网络应用程序Glycoanalysis by the ThreeAxes of MS and Chromatography (GALAXY)基于Noriko Takahashi博士开发的吡啶基-2-氨基化N-连接寡糖的三维HPLC图谱，已广泛用于分子、细胞和组织水平的N-糖基化分析。在此，我们将更新后的 GALAXY 描述为版本 3，其中包括新的 HPLC 数据（包括葡萄糖醛酸化和硫酸化聚糖的数据）、使用现代技术改进的图形用户界面，并链接到 GlyTouCan 和 GlyCosmos Portal 中的聚糖信息。这种联系将有助于结合多组学数据对人类和其他生物体进行糖组分析。
High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web applicationGlycoanalysis by the ThreeAxes of MS and Chromatography(GALAXY), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Herein, we describe the updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multiomics data.