Molecular epidemiology of the 2005 enterovirus 71 outbreak in central Taiwan
Molecular epidemiology of the 2005 enterovirus 71 outbreak in central Taiwan
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DOI：
10.3109/00365548.2010.545995
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发表时间：
2011-05-01
影响因子：
--
通讯作者：
Lin, Cheng-Wen
中科院分区：
文献类型：
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作者：
Lan, Yu-Ching;Lin, Tsai-Hsiu;Lin, Cheng-Wen
背景：自1998年以来，台湾每年都有肠道病毒71型(EV71)在全国范围内暴发。台中市周边地区在2005年经历了一次特别大的疫情，之后EV71型病毒消失了两年，直到2008年重新出现。在这里，我们介绍了2005年台中疫情的临床、基因分型和流行病学基线数据。方法：采集咽拭子、粪便和脑脊液标本，用病毒载体保存。标本用逆转录聚合酶链式反应和病毒培养进行检测。流行病学、实验室和临床数据取自病历回顾。共筛选出27株病毒分离株进行系统发育分析。结果：已确定的病毒系统发育结果分为5组。作为研究27株EV71分离株之间遗传关系的重点，这些EV71分离株都属于不同的C4基因分支。大部分毒株分属5个观察到的流行群。结论：2005年台湾中部暴发疫情是由属于C4基因的不同EV71菌株引起的。
Background: Since 1998, Taiwan has experienced annual outbreaks of enterovirus 71 (EV71) nationwide. The area around Taichung City experienced a particularly large outbreak in 2005, after which EV71 disappeared for 2 y before re-emerging in 2008. Here we present the clinical, genotypic, and epidemiological baseline data for the 2005 Taichung outbreak. Methods: Throat swab, stool and cerebrospinal fluid samples were collected and stored in viral transport medium. Samples were tested by reverse-transcriptase polymerase chain reaction and viral culture. Epidemiological, laboratory, and clinical data were extracted from medical record reviews. A total of 27 virus isolates were selected for phylogenetic analysis. Results: Confirmed phylogenetic results of the viruses were separated into 5 groups. The 5'-UTR regions served as a focus for investigating genetic relationships among the 27 EV71 isolates, all of which belonged to a distinct clade in the C4 genotype. Most of the strains belonged to 5 observed epidemic groups. Conclusion: In conclusion, the 2005 outbreak in central Taiwan was caused by divergent EV71 strains belonging to the C4 genotype.