Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus.
Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus.
复制标题
DOI：
10.1074/jbc.m113.502039
复制
发表时间：
2013-10-11
期刊：
影响因子：
--
通讯作者：
Schneewind O
中科院分区：
文献类型：
--
作者：
Chen C;Krishnan V;Macon K;Manne K;Narayana SV;Schneewind O
背景资料：表皮葡萄球菌分泌的蛋白酶Esp可阻断金黄色葡萄球菌生物膜的形成及其在人鼻孔的定植能力。结果：Esp能切割自溶素，从而阻止葡萄球菌DNA作为生物膜基质的释放。结论：分泌型蛋白酶控制S。金黄色葡萄球菌生物膜发育和宿主定植。意义：促进自溶素降解的方法也可以防止S。金黄色葡萄球菌在人类中的定植。表皮葡萄球菌（Staphylococcus epidermidis）是人类的一种寄生菌，分泌Esp蛋白酶以防止金黄色葡萄球菌生物膜的形成和定殖。封锁S。金黄色葡萄球菌定殖可降低侵袭性感染性疾病的发生率;然而，Esp破坏生物膜的机制尚不清楚。我们在这里表明，ESP切割自溶素（Atl）衍生的胞壁素水解酶，并防止葡萄球菌释放的DNA，作为细胞外基质中的生物膜。X射线晶体学分析表明，Esp的三维结构与S. aureus V8（SspA）。atl和sspA都是生物膜形成所必需的，并且纯化的SspA切割来源于大肠杆菌的胞壁蛋白水解酶。因此，S.金黄色葡萄球菌生物膜是通过自溶素的受控分泌和蛋白水解形成的，并且该发育程序似乎受到S.表皮
Background: Esp, a secreted protease of Staphylococcus epidermidis, blocks biofilm formation of Staphylococcus aureus and its ability to colonize human nares. Results: Esp cleaves autolysin, thereby preventing the release of staphylococcal DNA as biofilm matrix. Conclusion: Secreted proteases control S. aureus biofilm development and host colonization. Significance: Methods that promote autolysin degradation may also prevent S. aureus colonization of humans. Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis.