IRON-DEPENDENT FREE-RADICAL DAMAGE TO DNA AND DEOXYRIBOSE - SEPARATION OF TBA-REACTIVE INTERMEDIATES
IRON-DEPENDENT FREE-RADICAL DAMAGE TO DNA AND DEOXYRIBOSE - SEPARATION OF TBA-REACTIVE INTERMEDIATES
复制标题
DOI：
10.1016/0020-711x(82)90071-4
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
TOEG, D
中科院分区：
文献类型：
--
作者：
GUTTERIDGE, JMC;TOEG, D
铁依赖自由基对DNA和脱氧核糖的损伤导致硫代巴比妥酸（TBA）活性中间体的形成。这些中间体与黄嘌呤氧化酶和过氧化物酶孵育后用色谱法和分光光度法进行比较。在与黄嘌呤氧化酶孵育和与过氧化物酶孵育的丙二醛钠标准溶液中，博来霉素Fe（II）衍生产品发生了tba反应性的丧失。
Fe-dependent free radical damage to DNA and deoxyribose results in the formation of thiobarbituric acid (TBA) reactive intermediates. These intermediates were compared chromatographically and spectrophotometrically after incubation with the enzymes xanthine oxidase and peroxidase. Loss of TBA-reactivity occurred in the bleomycin Fe(II) derived products incubated with xanthine oxidase and in a standard solution of sodium malondialdehyde incubated with peroxidase.