Stress-derived reactive oxygen species enable hemocytes to release activator of growth blocking peptide (GBP) processing enzyme
Stress-derived reactive oxygen species enable hemocytes to release activator of growth blocking peptide (GBP) processing enzyme
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应激源性活性氧使血细胞释放生长阻断肽 (GBP) 加工酶的激活剂
DOI：
10.1016/j.jinsphys.2021.104225
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发表时间：
2021
影响因子：
2.2
通讯作者：
Hayakawa Yoichi
中科院分区：
文献类型：
--
作者：
Matsumoto Hitoshi;Ochiai Masanori;Imai Erina;Matsumura Takashi;Hayakawa Yoichi
昆虫细胞因子生长阻滞肽(GBP)是一种非活性前体，被称为proGBP，在非应激动物的血淋巴中通常存在大量的GBP(Hayakawa，1990，Hayakawa，1991)。在胁迫条件下，proGBP被丝氨酸蛋白酶瞬间降解为活性GBP，这被认为是昆虫通过GBP诱导的生理变化来应对应激诱导的不利影响的重要的第一步。然而，昆虫血淋巴proGBP在胁迫条件下蛋白质分解的详细机制仍不清楚。在这里，我们证明了proGBP的加工需要ROS诱导的一种蛋白质因子从血细胞中释放出来，激活失活的proGBP加工酶。激活蛋白从血细胞中的释放是由ROS诱导的胞浆内钙离子浓度升高所启动的。因此，我们得出结论，应激诱导的proGBP激活需要依赖于ROS刺激血细胞内的钙信号通路，然后释放特异性激活proGBP加工酶的血细胞蛋白因子。
Insect cytokine growth blocking peptide (GBP) is synthesized as an inactive precursor, termed proGBP, that is normally present in a significant concentration in the hemolymph of non-stressed animals (Hayakawa, 1990, Hayakawa, 1991). Under stress conditions, proGBP is instantly processed to active GBP by a serine protease and this is thought to be an important initial step for insects to cope with stress-induced adverse effects via GBP-induced physiological changes. However, the detailed mechanism underlying proteolytic processing of hemolymph proGBP in insects under stress conditions remains unknown. Here we demonstrated that proGBP processing requires ROS-induced release of a proteinaceous factor from hemocytes that activates the inactive proGBP processing enzyme. The release of the activator protein from hemocytes is initiated by an elevation of the cytoplasmic Ca2+concentration induced by ROS. Therefore, we concluded that stress-induced activation of proGBP requires ROS-dependent stimulation of an intracellular calcium signaling pathway in hemocytes, followed by release of the hemocyte proteinaceous factor that specifically activates the proGBP processing enzyme.