Human dental pulp stem cells derived from different cryopreservation methods of human dental pulp tissues of diseased teeth.
Human dental pulp stem cells derived from different cryopreservation methods of human dental pulp tissues of diseased teeth.
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DOI：
10.1111/j.1600-0714.2011.01040.x
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Lin LM
中科院分区：
文献类型：
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作者：
Chen YK;Huang AH;Chan AW;Shieh TY;Lin LM
人类牙髓干细胞（hDPSC）的成功分离已被证明在拔牙后至少120小时。活的hDPSC主要从冷冻保存的健康臼齿及其未消化的牙髓组织中分离。尚未报道在冷冻保存后从患病但有活力的牙齿中分离hDPSC。本研究旨在从冷冻保存的各种牙齿类型的患病但活的牙齿中分离hDPSCs。将50个牙标本分为A组（n = 20）新鲜牙髓组织、B组（n = 20）液氮保存牙髓组织和C组（n = 10）液氮保存完整牙。A组和B组的hDPSC分离成功率为100%，而C组仅为20%。来自所有组的hDPSC表现出自我更新特性和类似的成脂、成软骨和成骨分化的多潜能特性。此外，hDPSC显示出高表达的骨髓间充质干细胞标志物（CD29、CD90和CD105）和非常低表达的特异性造血细胞标志物（CD14、CD34和CD45）。我们的研究结果表明，从各种牙齿类型的患病但有活力的牙齿中分离的hDPSC可以储存在液氮中以供将来使用。
Successful isolation of human dental pulp stem cells (hDPSCs) has been documented at least 120 h after tooth extraction. Viable hDPSCs have been isolated chiefly from cryopreserved healthy molar teeth and their undigested dental pulp tissue. Isolation of hDPSCs from diseased but vital teeth after cryopreservation has not been reported. This study aimed to isolate hDPSCs from cryopreserved diseased but vital teeth of various tooth types. Fifty tooth samples were divided into group A (n = 20) – freshly derived dental pulp tissues, group B (n = 20) – liquid nitrogen (liq N2)-stored dental pulp tissues and group C (n = 10) – liq N2-stored intact teeth. The success rate for hDPSCs isolation was 100% for groups A and B and only 20% for group C. hDPSCs from all groups demonstrated self-renewal properties and similar multipotent potential characteristics of adipogenic, chondrogenic and osteogenic differentiation. In addition, hDPSCs showed high expression of bone-marrow mesenchymal stem-cell markers (CD29, CD90 and CD105) and very low expression of specific hematopoietic cells markers (CD14, CD34 and CD45). Our results indicate that hDPSCs isolated from diseased but vital teeth of various tooth types can be stored in liq N2 for future usage.