MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A RECEPTOR FOR PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE
MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A RECEPTOR FOR PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE
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DOI：
10.1016/0896-6273(93)90188-w
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发表时间：
1993-08-01
期刊：
影响因子：
16.2
通讯作者：
NAGATA, S
中科院分区：
文献类型：
--
作者：
HASHIMOTO, H;ISHIHARA, T;NAGATA, S
垂体腺苷酸环化酶激活多肽（PACAP）是一种与血管活性肠多肽（VIP）相关的多肽激素。通过与大鼠 VIP 受体 cDNA 交叉杂交，从脑 cDNA 文库中分离出大鼠 PACAP 受体 cDNA。 COS细胞中表达的重组PACAP受体与PACAP的结合亲和力比VIP高约1000倍，并且PACAP通过克隆的PACAP受体刺激腺苷酸环化酶。大鼠PACAP受体由495个氨基酸组成，包含7个跨膜片段，与其他G(s)偶联受体如VIP、胰高血糖素和促胰液素受体具有显着相似性。 PACAP受体mRNA在大脑中大量表达，但在除肾上腺外的外周组织中不表达。原位杂交显示海马齿状回、嗅球和小脑皮质中 PACAP 受体 mRNA 高水平表达。
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a polypeptide hormone related to vasoactive intestinal polypeptide (VIP). Rat PACAP receptor cDNA was isolated from a brain cDNA library by cross-hybridization with rat VIP receptor cDNA. The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000 times higher affinity than VIP, and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The rat PACAP receptor consists of 495 amino acids, contains seven transmembrane segments, and has a significant similarity with other G(s)-coupled receptors, such as VIP, glucagon, and secretin receptors. PACAP receptor mRNA was abundantly expressed in the brain, but not in the peripheral tissues except for the adrenal gland. In situ hybridization revealed a high level of expression of PACAP receptor mRNA in the hippocampal dentate gyrus, olfactory bulb, and cerebellar cortex.