Water-soluble photoluminescent fullerene capped mesoporous silica for pH-responsive drug delivery and bioimaging
Water-soluble photoluminescent fullerene capped mesoporous silica for pH-responsive drug delivery and bioimaging
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DOI：
10.1088/0957-4484/27/31/315104
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发表时间：
2016-06
期刊：
影响因子：
3.5
通讯作者：
Lei Tan;Tao Wu;Zhao-Wen Tang;Jiangrong Xiao;R. Zhuo;B. Shi;Chuan-Jun Liu
中科院分区：
文献类型：
--
作者：
Lei Tan;Tao Wu;Zhao-Wen Tang;Jiangrong Xiao;R. Zhuo;B. Shi;Chuan-Jun Liu
本文成功制备了一种生物相容性和水溶性的富勒烯（C60-TEG-COOH）包覆的介孔二氧化硅纳米颗粒（MSN），用于pH敏感的药物释放和荧光细胞成像。首先将MSN与3-氨丙基三乙氧基硅烷反应得到氨基改性的MSN，然后在PBS溶液（pH = 7.4）中用带有羧基的水溶性C60通过与氨基的静电作用覆盖MSN表面。在弱酸性环境（溶酶体，pH = 5.0）中，由于C60-TEG-COO-的质子化，导致C60-TEG-COOH修饰的MSN（MSN@C60）解离，从而引发盐酸阿霉素（DOX）的释放。此外，由于C60修饰的MSN的绿色荧光性质，可以跟踪细胞对纳米颗粒的摄取。在体外研究中，所制备的材料显示出良好的生物相容性和阿霉素负载的纳米载体表现出有效的抗癌能力。本工作为设计同时具有pH响应的药物递送和生物成像系统提供了一种简单的方法。
In this paper, a biocompatible and water-soluble fluorescent fullerene (C60-TEG-COOH) coated mesoporous silica nanoparticle (MSN) was successfully fabricated for pH-sensitive drug release and fluorescent cell imaging. The MSN was first reacted with 3-aminopropyltriethoxysilane to obtain an amino-modified MSN, and then the water-soluble C60 with a carboxyl group was used to cover the surface of the MSN through electrostatic interaction with the amino group in PBS solution (pH = 7.4). The release of doxorubicin hydrochloride (DOX) could be triggered under a mild acidic environment (lysosome, pH = 5.0) due to the protonation of C60-TEG-COO−, which induced the dissociation of the C60-TEG-COOH modified MSN (MSN@C60). Furthermore, the uptake of nanoparticles by cells could be tracked because of the green fluorescent property of the C60-modified MSN. In an in vitro study, the prepared materials showed excellent biocompatibility and the DOX-loaded nanocarrier exhibited efficient anticancer ability. This work offered a simple method for designing a simultaneous pH-responsive drug delivery and bioimaging system.