Electrical stimulation-induced seizures in rats: A "dose-response" study on resultant neurodegeneration
Electrical stimulation-induced seizures in rats: A "dose-response" study on resultant neurodegeneration
复制标题
DOI：
10.1111/j.1528-1167.2011.03159.x
复制
发表时间：
2011-09-01
期刊：
影响因子：
5.6
通讯作者：
Rosenow, Felix
中科院分区：
文献类型：
--
作者：
Norwood, Braxton A.;Bauer, Sebastian;Rosenow, Felix
穿孔通路刺激（PPS）用于研究啮齿动物的颞叶癫痫。高频率PPS诱发急性癫痫发作，可导致神经元死亡和自发性癫痫。然而，在未处理啮齿动物中诱导神经变性的PPS的最短持续时间尚不清楚。自由活动的Sprague-Dawley大鼠接受一次连续双侧PPS（范围1-180 min）。海马颗粒细胞层的同步记录证实了癫痫样活动的存在，并精确地显示了癫痫发作活动何时被麻醉终止。PPS后1-7天，Fluoro-Jade B染色确定神经元变性。35分钟的连续PPS在大脑任何地方都没有产生明显的神经元死亡。这些数据表明，在自由移动的幼稚大鼠：（1）40分钟的PPS诱导的癫痫发作活动是脑细胞死亡的阈值，（2）齿状门神经元是最容易受到PPS。进一步的研究是必要的，以确定癫痫性神经变性的阈值。
Perforant pathway stimulation (PPS) is used to study temporal lobe epilepsy in rodents. High-frequency PPS induces acute seizures, which can lead to neuron death and spontaneous epilepsy. However, the minimum duration of PPS that induces neurodegeneration in naive rodents is unknown. Freely moving Sprague-Dawley rats received one episode of continuous, bilateral PPS (range 1-180 min). Simultaneous recording from the hippocampal granule cell layer confirmed the presence of epileptiform activity and showed precisely when seizure activity was terminated by anesthesia. Fluoro-Jade B staining, 1-7 days after PPS, determined neuronal degeneration. Thirty-five minutes of continuous PPS produced no apparent neuron death anywhere in the brain. The minimum duration that caused neurodegeneration, which was confined to the dentate hilus, was 40 min. These data indicate that, in freely moving naive rats: (1) 40 min of PPS-induced seizure activity is the threshold for brain cell death, and (2) dentate hilar neurons are the most vulnerable to PPS. Further studies are warranted to determine the threshold of epileptogenic neurodegeneration.