Phospholipid metabolism in calcium-regulated differentiation in cultured murine keratinocytes.
Phospholipid metabolism in calcium-regulated differentiation in cultured murine keratinocytes.
复制标题
培养的鼠角质形成细胞中钙调节分化中的磷脂代谢。
DOI：
10.1016/0006-291x(84)91224-5
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Pandey,R
中科院分区：
文献类型：
--
作者：
Ziboh,VA;Isseroff,RR;Pandey,R
目前的研究结果关联磷脂的改变，特别是磷脂酰肌醇的营业额，在钙离子诱导的角质形成细胞的分化。这些结论是基于预先标记的PI水解14 C-AA以及细胞从低浓度到正常浓度的细胞外Ca ~（2+）转换后14 C-DG和14 C-PA的积累。这一新的发现意味着，伴随角质形成细胞分化后，从低切换到正常的细胞外介质的生物学变化可能是由于至少部分增加的PA和DG的积累，这是主要的磷脂酰肌醇的脱酰和再酰化产物。在这些研究中的第二个有趣的发现是，当与在正常Ca 2+中培养的分化细胞相比时，在低Ca 2+培养基中培养的增殖角质形成细胞将14 C-AA显著转化为脂氧合酶产物。脂肪氧合酶产物生成减少在表皮分化中的意义值得进一步探讨。
The present findings associate phospholipid alteration, particularly the turnover of phosphatidylinositol, in Ca2+induced differentiation of keratinocytes. These conclusions are based on the hydrolysis of14C-AA from prelabeled PI and the accumulation14C-DG and14C-PA after cells are switched from low to normal concentrations of extracellular Ca2+. This novel finding implies that the biological changes which accompany keratinocyte differentiation after switch from low to normal extracellular medium may be due at least in part to increased accumulation of PA and DG which are major deacylation and reacylation products of phosphatidylinositol. A second interesting finding in these studies is the marked transformation of14C-AA into lipoxygenase products by proliferating keratinocytes cultured in low Ca2+medium when compared to differentiating cells cultured in normal Ca2+. The significance of decreased generation of lipoxygenase products in epidermal differentiation deserve further exploration.