Influenza virus site recognized by a murine helper T cell specific for H1 strains. Localization to a nine amino acid sequence in the hemagglutinin molecule.
Influenza virus site recognized by a murine helper T cell specific for H1 strains. Localization to a nine amino acid sequence in the hemagglutinin molecule.
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流感病毒部位被特异性H1菌株的鼠辅助T细胞识别。在血凝素分子中定位到九个氨基酸序列。
DOI：
10.1084/jem.158.2.294
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发表时间：
1983-08-01
期刊：
影响因子：
--
通讯作者：
Melchers F
中科院分区：
文献类型：
--
作者：
Hackett CJ;Dietzschold B;Gerhard W;Ghrist B;Knorr R;Gillessen D;Melchers F
功能性辅助性T细胞系Vir-2来源于PR 8（H1N1）流感病毒免疫的BALB/c小鼠，对同源抗原呈递细胞和来自1934-1957和1977-1980分离株的H1亚型人流感病毒的天然存在株作出应答而增殖。重链（HA 1）中氨基酸位置115周围的血凝素分子保守区在这种识别中是重要的，因为在源自野生型PR 8的实验室突变体RV 6中，缺乏与位置115处的谷氨酸至赖氨酸取代相关的刺激活性。然后使用切割产物和合成肽对野生型血凝素分子上的刺激决定簇进行表征。Vir-2细胞识别PR 8病毒的还原和烷基化纯化HA 1，并且在甲硫氨酸和色氨酸残基处裂解后保留这种反应性。裂解片段的高压液相色谱分离表明，含有残基115的HA 1的短序列被识别。通过测定来自该区域的不同长度的合成肽同系物的刺激，将这种识别定位于9个氨基酸区段（位置111-119）。与天然血凝素一样，Vir-2细胞在H-2d抗原呈递细胞而不是H-2k抗原呈递细胞呈递时对活性肽有反应。
The functional helper T cell line Vir-2, derived from a PR8 (H1N1) influenza virus-immunized BALB/c mouse, proliferates in response to syngeneic antigen-presenting cells and naturally occurring strains of subtype H1 human influenza virus from 1934-1957 and 1977-1980 isolates. A conserved region of the hemagglutinin molecule around amino acid position 115 in the heavy chain (HA1) was implicated as being important in this recognition by the lack of stimulatory activity associated with a glutamic acid to lysine substitution at position 115 in the laboratory mutant RV6, derived from wild-type PR8. Characterization of the stimulatory determinant on the wild-type hemagglutinin molecule was then undertaken using cleavage products and synthetic peptides. Vir-2 cells recognized the reduced and alkylated purified HA1 of PR8 virus, and this reactivity was retained after cleavage at methionine and tryptophan residues. High-pressure liquid chromatography separation of cleavage fragments indicated that a short sequence of the HA1 containing residue 115 was being recognized. This recognition was localized to a nine amino acid segment (positions 111-119) by assaying stimulation with synthetic peptide homologues of different lengths from that region. As with native hemagglutinin, Vir-2 cells responded to active peptides when presented by H-2d but not H-2k antigen-presenting cells.