Morphology and Enzymatic Degradation of Solution-Grown Single Crystals of Poly(ethylene succinate)
Morphology and Enzymatic Degradation of Solution-Grown Single Crystals of Poly(ethylene succinate)
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DOI：
10.1021/ma010865c
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发表时间：
2001-09
期刊：
影响因子：
5.5
通讯作者：
T. Iwata;Y. Doi;Kei Isono;Y. Yoshida
中科院分区：
文献类型：
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作者：
T. Iwata;Y. Doi;Kei Isono;Y. Yoshida
以0.025%的氯苯溶液为原料，在等温结晶条件下制备了聚丁二酸乙二醇酯（PES）层状单晶。在不同的晶化温度下，得到了有螺旋生长和无螺旋生长的菱形片晶。晶体产生了分辨良好的电子衍射图。聚乙烯装饰的晶体导致在一个“跨部门”的表面形态，并表明，平均方向的链折叠是平行的晶体生长平面。在某些情况下，层状晶体显示出螺旋生长起源于螺旋位错与左手和右手的形式。从粪产碱菌T1和施氏假单胞菌YM1006中分离纯化的两种胞外聚羟基丁酸酯（PHB）解聚酶用于PES层状晶体的酶促降解。利用免疫金标记技术观察到两种胞外PHB解聚酶在晶体表面的吸附。那个...
Solution-grown lamellar single crystals of poly(ethylene succinate) (PES) were prepared from a 0.025% solution of monochlorobenzene under an isothermal crystallization condition. Lozenge-shaped lamellar crystals with and without spiral growth were obtained at different crystallization temperature. The crystals produced well-resolved electron diffractograms. Polyethylene decoration of the crystals resulted in a “cross-sector” surface morphology and showed that the average direction of chain-folding is parallel to the crystal growth planes. In some cases, the lamellar crystals showed spiral growths originating from screw dislocations with both left-handed and right-handed forms. Two extracellular poly(hydroxybutyrate) (PHB) depolymerases purified from Alcaligenes faecalis T1 and Pseudomonas stutzeri YM1006 were used for the enzymatic degradation of the PES lamellar crystals. Adsorption of both the extracellular PHB depolymerases onto the crystal surface was observed using immuno-gold labeling technique. The...