Inactivation kinetics of model and relevant blood-borne viruses by treatment with sodium hydroxide and heat
Inactivation kinetics of model and relevant blood-borne viruses by treatment with sodium hydroxide and heat
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DOI：
10.1006/biol.1998.0146
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发表时间：
1998-09-01
期刊：
影响因子：
1.7
通讯作者：
Uren, E
中科院分区：
文献类型：
--
作者：
Borovec, S;Broumis, C;Uren, E
为了确定原位清洁系统灭活人血浆中病毒的有效性，研究了60 ℃下0.1 M氢氧化钠对病毒感染性的影响。以下模型和相关病毒的灭活随时间变化：人甲型肝炎病毒（HAV）、犬细小病毒（CPV;人细小病毒B-19的模型）、伪狂犬病病毒（PRV，肝炎B病毒的模型）和牛病毒性腹泻病毒（BVDV，丙型肝炎病毒和人免疫缺陷病毒的模型）。通过一种新型原位EIA方法测定了CPV的感染性，该方法将证明可用于验证细小病毒灭活或清除的研究，证明BVDV、PRV和CPV的感染性在60 ℃下用0.1 M NaOH在30 s内可重复灭活至检测限以下。HAV灭活低于检测限2分钟内。单独加热处理也导致一些对数减少所有病毒测试，但CPV在60 ℃加热16分钟后保持不受影响。HAV单独处理氢氧化物（高达1.0 M）在15 ℃不会导致快速灭活。总之，这些数据表明，0.1 M NaOH在60 ℃下持续2 min应足以杀灭工艺残留物中存在的病毒。(C)1998年，国际生物标准化协会。
To determine the efficacy of a clean-in-place system for the inactivation of viruses present in human plasma, the effect of 0.1 M sodium hydroxide at 60 degrees C on viral infectivity was investigated. Inactivation of the following model and relevant viruses were followed as a function of time: human hepatitis A virus (HAV), canine parvovirus (CPV; a model for human parvovirus B-19) pseudorabies virus (PRV, a model for hepatitis B virus), and bovine viral diarrhoea virus (BVDV, a model for hepatitis C virus and human immunodeficiency virus). Infectivity of CPV was determined by a novel in situ EIA method which will prove useful for studies to validate parvovirus inactivation or removal, infectivity of BVDV, PRV and CPV were shown to be reproducibly inactivated below the limit of detection by 0.1 M NaOH at 60 degrees C within 30 s. HAV was inactivated to below the limit of detection within 2 min. Treatment with heat alone also resulted in some log reduction for all Viruses tested except for CPV which remained unaffected after heating at 60 degrees C for 16 min. Treatment of HAV with hydroxide alone (up to 1.0 M) at 15 degrees C did not lead to rapid inactivation. Collectively, these data suggest that 0.1 M NaOH at 60 degrees C for two min should be sufficient to inactivate Viruses present in process residues. (C) 1998 The International Association of Biological Standardization.