The fluorescently responsive 3-(naphthalen-1-ylethynyl)-3-deaza-2'-deoxyguanosine discriminates cytidine via the DNA minor groove
The fluorescently responsive 3-(naphthalen-1-ylethynyl)-3-deaza-2'-deoxyguanosine discriminates cytidine via the DNA minor groove
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荧光响应的 3-(萘-1-乙炔基)-3-deaza-2-脱氧鸟苷通过 DNA 小沟区分胞苷
DOI：
10.1039/c7ob01931a
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y. Saito*
中科院分区：
文献类型：
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作者：
A. Suzuki;M. Yanagi;T. Takeda;R. H. E. Hudson;Y. Saito*
合成了一种新的环境响应型荧光核苷3-（萘-1-基乙炔基）-3-脱氮-2 ′-脱氧鸟苷（3 nzG）。核苷，3 nzG，表现出溶剂化显色特性，当引入到ODN探针中时，它能够通过探测DNA小沟中微环境的变化，通过其发射波长的明显变化识别靶链中的2′-脱氧胞苷。因此，3 nzG具有用作荧光探针分子的潜力，用于核酸的微观结构研究，包括检测靶DNA序列中的单碱基改变。
A new environmentally responsive fluorescent nucleoside, 3-(naphthalen-1-ylethynyl)-3-deaza-2′-deoxyguanosine (3nzG), has been synthesized. The nucleoside, 3nzG, exhibited solvatochromic properties and when introduced into ODN probes it was able to recognize 2′-deoxycytidine in target strands by a distinct change in its emission wavelength through probing microenvironmental changes in the DNA minor groove. Thus, 3nzG has the potential for use as a fluorescent probe molecule for micro-structural studies of nucleic acids including the detection of single-base alterations in target DNA sequences.