Development of an antibacterial chitin betainate wound dressing
Development of an antibacterial chitin betainate wound dressing
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DOI：
10.1038/pj.2014.47
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发表时间：
2014-08-01
期刊：
影响因子：
2.8
通讯作者：
Pinyakong, Onruthai
中科院分区：
文献类型：
--
作者：
Tachaboonyakiat, Wanpen;Sukpaiboon, Ekkachai;Pinyakong, Onruthai
本研究涉及化学改性甲壳素与带正电荷的基团，赋予抗菌活性。甲壳素甜菜碱酸酯是甲壳素的一种季铵盐，由甲壳素与羧甲基三甲基氯化铵反应制得。通过傅里叶变换红外光谱（FTIR）解析了化学结构，其显示在1738 cm（-1）处存在额外的酯峰，并且在1650 cm（-1）处酰胺I发生了一些变化。制备了甲壳素和甲壳素甜菜碱凝胶，并对其吸水率、接触角和抗菌活性进行了测试。甲壳素甜菜酸酯具有比甲壳素高1.07倍的吸水水平和更高的亲水性，并且它还表现出对金黄色葡萄球菌和大肠杆菌的抗菌活性。在10 mg ml（-1）的终浓度下，甲壳素甜菜碱能完全杀死大肠杆菌.大肠杆菌，S.金黄色葡萄球菌在10 min和24 h后分别减少45.2%和78%。对S.金黄色葡萄球菌呈剂量依赖性，而对E.当使用甲壳素甜菜碱酸盐时.
This research involved the chemical modification of chitin with positively charged groups to confer antibacterial activity. Chitin betainate, a quaternary ammonium chitin, was prepared by the acylation of chitin with carboxymethyl trimethyl ammonium chloride. The chemical structure was elucidated by Fourier transform infrared spectroscopy (FTIR), which revealed the presence of the additional ester peak at 1738 cm(-1) and some change in amide I at 1650 cm(-1). Chitin and chitin betainate gels were prepared and then tested for their water absorption, contact angle and antibacterial activity. The chitin betainate had a 1.07-fold higher water-absorption level and a higher hydrophilic property than chitin, and it also exhibited an antibacterial activity against Staphylococcus aureus and Escherichia coli. At a final concentration of 10 mg ml(-1), chitin betainate completely killed E. coli within 10 mm, and S. aureus was reduced by 45.2% and 78% after 10 min and 24 h, respectively. The antibacterial activity against S. aureus was dose-dependent, whereas no antibacterial activity was observed against E. coli when using chitin betainate at