Regulation of the integration-excision reaction by bacteriophage lambda.
Regulation of the integration-excision reaction by bacteriophage lambda.
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噬菌体 lambda 调节整合-切除反应。
DOI：
10.1101/sqb.1981.045.01.058
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Sosa,L
中科院分区：
文献类型：
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作者：
Miller,HI;Abraham,J;Benedik,M;Campbell,A;Court,D;Echols,H;Fischer,R;Galindo,JM;Guarneros,G;Hernandez,T;Mascarenhas,D;Montanez,C;Schindler,D;Schmeissner,U;Sosa,L
噬菌体h调节整合-切除反应，这是沿着溶原性和产生性两条病毒途径受控发展的关键方面。溶原性反应的基本事件是生产功能的完全抑制和病毒DNA的整合。对生产性反应至关重要的事件是复制和成熟功能的有序表达，对于诱导的原噬菌体来说，是病毒DNA的切除。噬菌体可利用的两条途径之间的选择可以根据最初对一条途径或另一条途径的调节划分来考虑，随后的调节事件反过来又稳定了这两条途径。的CLI和CLII基因产物(CLI和CLII蛋白)提供溶原性反应的第一阶段，启动维持抑制物Ci和整合蛋白Int的产生，并延迟晚期裂解功能的表达。Ci蛋白通过切断受~cro和q产物(Cro和Q)调节的产生途径来稳定溶源途径；超过切除蛋白Xis的Int过量执行向插入的重组开关(Herskowitz 1973；Weisberg等人审查)。1977年；Echols1979,1980；图1)。选择原生或繁殖性生长与寄主和噬菌体有关。CLI蛋白可能是主要的分配功能，其他调控影响也会对其产生影响。其中包括噬菌体蛋白clII和宿主蛋白HFL和HIMA。由于HIMA也是整合重组中Int活性的辅助因子，因此在整合重组的活性和合成水平上存在整合重组的双重调节，整合和切除的定向控制的基础是正反向反应的不同催化要求。如下图所示，整合重组所需的唯一噬菌体特异性蛋白是Int，而激活性重组需要病毒蛋白Int和Xis(Weisberg等人。1977年；纳什1978年；回声1980年)。
Bacteriophage h regulates the integration-excision reaction as a crucial aspect of controlled development along the two viral pathways, lysogenic and productive. The events essential for the lysogenic response are complete repression of productive functions and integration of the viral DNA. The events essential for the productive response are an ordered expression of replication and maturation functions and, for an induced prophage, excision of the viral DNA. The choice between the two pathways available to the phage can be considered in terms of an initial regulatory partition toward one pathway or the other, stabilized in turn by subsequent regulatory events. The clI-and clII-gene products (clI and clII proteins) of)~ provide for the first stage of the lysogenic response, turning on production of the maintenance repressor cI and the integration protein Int and delaying the expression of late lytic functions. The cI protein stabilizes the lysogenic pathway by a switchoff of the productive pathway regulated by the~ cro and Q products (Cro and Q); an excess of Int over the excision protein Xis executes the recombinational switch toward insertion (reviewed by Herskowitz 1973; Weisberg et al. 1977; Echols 1979, 1980; Fig. 1). The choice oflysogenic or productive growth involves the host as well as the phage. The clI protein is probably the primary partition function on which other regulatory influences act. These include the phage protein clII and host proteins Hfl and HimA. Since HimA is also an accessory factor for the activity of Int in integrative recombination, there exists dual regulation of integrative recombination at the level of activity and synthesis of Int.The basis for directional control of integration and excision is the different catalytic requirements for the forward and reverse reactions. As diagramed below, the only phage-specified protein required for integrative recombination is Int, whereas excisive recombination requires the viral proteins Int and Xis (Weisberg et al. 1977; Nash 1978; Echols 1980).