Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells
Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells
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DOI：
10.1371/journal.pone.0227174
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发表时间：
2020-01-03
期刊：
影响因子：
3.7
通讯作者：
Yuan, Bao-Zhu
中科院分区：
文献类型：
--
作者：
Han, Xiaoyan;Na, Tao;Yuan, Bao-Zhu
BEAS-2B最初是从人的支气管上皮细胞中建立的永生化但不会致癌的上皮细胞系。由于BEAS-2B细胞系具有广泛的支气管上皮来源，已被广泛用于包括肺癌发生在内的多种呼吸系统疾病的体外细胞模型研究中。然而，很少有研究讨论BEAS-2B细胞的非上皮性特征，特别是与间充质干细胞(MSCs)相关的特征，MSCs代表着一组自我更新和向不同细胞系分化的成纤维细胞样细胞。在这项研究中，我们比较了BEAS-2B和作为hMSCs代表的人脐血间充质干细胞(HMSCs)细胞系hMSCs的共同特征。观察到BEAS-2B和hMSC1具有相同的hMSCs表面标志的表达谱，并显示出相似的成骨和成脂分化潜能。此外，与hMSC1一样，BEAS-2B细胞株对丝裂原激活的总T细胞和Th1细胞的增殖以及干扰素-γ诱导的IDO1的表达均表现出抑制作用，表明BEAS-2B细胞与hMSCs表现出几乎相同的特征特征，尽管在对2型巨噬细胞极化的影响上BEAS-2B与hMSCs有明显的差异。最重要的是，BEAS-2B的hMSCs特征不太可能是上皮间充质转化的结果。因此，这项研究为重新考虑BEAS-2B的上皮起源提供了一组证据。
BEAS-2B was originally established as an immortalized but non-tumorigenic epithelial cell line from human bronchial epithelium. Because of general recognition for its bronchial epithelial origin, the BEAS-2B cell line has been widely used as an in vitro cell model in a large variety of studies associated with respiratory diseases including lung carcinogenesis. However, very few studies have discussed non-epithelial features of BEAS-2B cells, especially the features associated with mesenchymal stem cells (MSCs), which represent a group of fibroblast-like cells with limited self-renewal and differentiation potential to various cell lineages. In this study, we compared BEAS-2B with a human umbilical cord-derived MSCs (hMSCs) cell line, hMSC1, which served as a representative of hMSCs in terms of expressing common features of hMSCs. It was observed that both BEAS-2B and hMSC1 shared the same expression profile of surface markers of hMSCs and exhibited similar osteogenic and adipogenic differentiation potential. In addition, like hMSC1, the BEAS-2B cell line exhibited suppressive activities on proliferation of mitogen-activated total T lymphocytes as well as Th1 lymphocytes, and IFN gamma-induced expression of IDO1, all thus demonstrating that BEAS-2B cells exhibited an almost identical characteristic profile with hMSCs, even though, there was a clear difference between BEAS-2B and hMSCs in the effects on type 2 macrophage polarization. Most importantly, the hMSCs features of BEAS-2B were unlikely a consequence of epithelial-mesenchymal transition. Therefore, this study provided a set of evidence to provoke reconsideration of epithelial origin of BEAS-2B.