A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode
A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode
复制标题
基于多层碳纳米管修饰电极上逐层组装的葡萄糖氧化酶直接电化学的灵敏稳定的生物传感器
DOI：
10.1016/j.bios.2010.06.013
复制
发表时间：
2010-09-15
影响因子：
12.6
通讯作者：
Si, Shihui
中科院分区：
文献类型：
--
作者：
Deng, Chunyan;Chen, Jinhua;Si, Shihui
A novel strategy for fabricating the sensitive and stable biosensor was present by layer-by-layer (LBL) self-assembling glucose oxidase (GOD) on multiwall carbon nanotube (CNT)-modified glassy carbon (GC) electrode GOD was immobilized on the negatively charged CNT surface by alternatively assembling a cationic poly(ethylenimine) (PEI) layer and a GOD layer And the direct electrochemistry of GOD in the self-assembled {GOD/PEI}(n) film was investigated CNT as an excellent nanomatenal greatly improved the direct electron transfer between GOD in (GOD/PEI}(n) film and the electrode And the ultrathin {GOD/PEI}(n) film on the CNT surface provided a favorable microenvironment to keep the bioactivity of GOD Moreover, PEI used as an out-layer was adsorbed on the top of the (GOD/PEI}(n) film to form the sandwich-like structure (PEI/{GOD/PEI}(n)), improving the stability of the enzyme electrode On basis of these, the developed PEI/{GOD/PEI}(n)/CNT/GC biosensor has a high sensitivity of 106 57 mu A mM(-1) cm(-2), and can measure as low as 0 05 mM glucose In addition, the biosensor has excellent operational stability with no decrease in the activity of enzyme over a 1-week period. Therefore, the developed strategy making use of the advantages of CNT and LBL assembly is ideal for the direct electrochemistry of the redox enzymes and the construction of the sensitive and stable enzyme biosensor (C) 2010 Elsevier B V All rights reserved
