Increased expression of nuclear factor E2 P45-related factor 2 (NRF2) in head and neck squamous cell carcinomas
Increased expression of nuclear factor E2 P45-related factor 2 (NRF2) in head and neck squamous cell carcinomas
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DOI：
10.1002/hed.20430
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发表时间：
2006-09-01
影响因子：
2.9
通讯作者：
Freeman, Michael L.
中科院分区：
文献类型：
--
作者：
Stacy, Donnie R.;Ely, Kim;Freeman, Michael L.
背景头颈部鳞状细胞癌（HNSCC）持续引起显著的发病率和死亡率。特异性11期基因产物的过表达可能代表重要的生物标志物。11期基因表达的一个调节因子是转录因子核因子E2 p45相关因子2（Nrf2）。评估HNSCC中Nrf2的表达，以确定其是否可作为疾病早期检测的生物标志物。由来自47个HNSCC的141个HNSCC活检芯构建组织微阵列。此外，从7例患者中获得了组织学正常的鳞状粘膜。蛋白质表达通过荧光化学进行评价。91.5%的肿瘤中Nrf2表达增加。硫氧还蛋白（一种Nrf2调节的基因产物）的表达在75%的肿瘤中升高。Keap1调节Nrf2泛素化和降解的速率，与正常粘膜相比，在HNSCC中相对过表达。Nrf2表达可能是HNSCC的候选生物标志物。(c)2006 Wiley Periodicals，Inc.
Background. Head and neck squamous cell carcinoma (HNSCC) continues to cause significant morbidity and mortality. Overexpression of specific phase 11 gene products may represent an important biomarker. One regulator of phase 11 gene expression is the transcription factor nuclear factor E2 p45-related factor 2 (Nrf2). Nrf2 expression was evaluated in HNSCC, to determine whether it might serve as a biomarker for early detection of disease.Methods. A tissue microarray was constructed of 141 HNSCC biopsy cores from 47 HNSCCs. In addition, histologically normal squamous mucosa was obtained from 7 patients. Protein expression was evaluated by immunohistochemistry.Results. Nrf2 expression was increased in 91.5% of tumors. Expression of thioredoxin, a Nrf2-reguated gene product, was elevated in 75% of tumors. Keap1, which regulates the rate of Nrf2 ubiquitination and degradation, was relatively overexpressed in HNSCC compared with normal mucosa.Conclusions. Nrf2 expression may be a possible HNSCC candidate biomarker. (c) 2006 Wiley Periodicals, Inc.