Analysis of competing endogenous RNAs and microRNAs in tea (Camellia sinensis) leaves during infection by the leaf spot pathogen, Pestalotiopsis trachicarpicola
Analysis of competing endogenous RNAs and microRNAs in tea (Camellia sinensis) leaves during infection by the leaf spot pathogen, Pestalotiopsis trachicarpicola
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叶斑病原菌 Pestalotiopsis trachicarpicola 感染期间茶树 (Camellia sinensis) 叶子中竞争性内源 RNA 和 microRNA 的分析
DOI：
10.1094/mpmi-10-21-0262-a
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发表时间：
2022
影响因子：
3.5
通讯作者：
Chen Z.
中科院分区：
文献类型：
--
作者：
Yang Y. Q.;Yin Q. X.;Qiu C. L.;Xia Z. Q.;Huang H. K.;Huang C.;Jiang X. Y.;Yang Y. Y.;Wang D. L.;Chen Z.
拟盘多毛孢（pstalotiopsis trachicarpicola YM Zhang & KD Hyde）是一种植物致病性真菌，可引起茶树叶斑病，严重降低茶叶产量和品质。相互竞争的内源性rna （ceRNAs）包括信使rna （mrna）、环状rna （circRNAs）和长链非编码rna (lncRNAs)，它们可以通过与microRNAs （miRNAs）中的应答元件结合来调节基因表达，而microRNAs （miRNAs）反过来又对靶mrna进行转录后调控。在本研究中，来自侵染茶（C. sinensis var. sinensis cv.）和未侵染茶（C. sinensis cv.）叶片的cerna和mirna进行了比较。对福丁-大白茶)进行测序和分析，预测单个cerna与mirna之间的相互作用，并鉴定靶基因。在ceRNA相互作用对中，有31对、24对、6对和2对分别以miRNA-mRNA、miRNA-lncRNA、miRNA-circRNA和mRNA-lncRNA相互作用的形式预测。预计在lncRNA/circRNA-miRNA-mRNA调控对中会发生三种类型的相互作用：i) circRNA-miRNA-mRNA（类型1相互作用），ii) lncRNA- mirna - mrna（类型2相互作用），iii) circRNA-lncRNA-miRNA-mRNA（类型3相互作用）。感染茶叶中lncRNA/circRNA-miRNA-mRNA中的一些互作对最终改变了mrna的丰度，如TEA015316。1: TEA015316。1, TEA020085。1: TEA020085。1, TEA027239。1: TEA027239。1、TEA001447。1: TEA001447。1. 分析茶树感染过程中产自茶叶的cerna和mirna的类型、表达丰度及其相互作用关系，将为进一步研究这种宿主-病原体相互作用提供有价值的资源。茶（Camellia sinensis）O. Kuntze]是一种多年生、常绿、小乔木或灌木，是亚洲热带和亚热带地区种植的重要经济作物（Li et al. 2019b）。由于它的多年生性质，种植面积广泛，茶园的生态环境复杂，茶叶遭受许多疾病，很大程度上
Pestalotiopsis trachicarpicola YM Zhang & KD Hyde is a plant-pathogenic fungus that can cause leaf spot on tea (Camellia sinensis), seriously decreasing the production and quality of tea leaves. Competing endogenous RNAs (ceRNAs) include messenger RNAs (mRNAs), circular RNAs (circRNAs), and long noncoding RNAs (lncRNAs), which can regulate gene expression through binding to response elements in microRNAs (miRNAs), which, in turn, operate posttranscriptional regulation of target mRNAs. In the current study, ceRNAs and miRNAs originating from infected and uninfected leaves of tea (C. sinensis var. sinensis cv. Fuding-dabaicha) were sequenced and analyzed, and the interaction between individual ceRNAs and miRNAs was predicted, with target genes being identified. Of the ceRNA interaction pairs, 31, 24, six, and two were predicted in the form of miRNA-mRNA, miRNA-lncRNA, miRNA-circRNA, and mRNA-lncRNA interactions, respectively. Three types of interactions were predicted to occur in the regulation pairs of lncRNA/circRNA-miRNA-mRNA: i) circRNA-miRNA-mRNA (type 1 interactions), ii) lncRNA-miRNA-mRNA (type 2 interactions), and iii) circRNA-lncRNA-miRNA-mRNA (type 3 interactions). Some interaction pairs in lncRNA/circRNA-miRNA-mRNA in infected tea leaves ultimately changed the abundance of mRNAs, such as TEA015316. 1: TEA015316. 1, TEA020085. 1: TEA020085. 1, TEA027239. 1: TEA027239. 1, and TEA001447. 1: TEA001447. 1. Analysis of the types and expression abundance of ceRNAs and miRNAs originating from tea leaves during infection by P. trachicarpicola, and their interactive relationship will provide a valuable resource for future research into this host-pathogen interaction. Tea [Camellia sinensis (L.) O. Kuntze] is a perennial, evergreen, small tree or shrub and is an important cash crop grown in plantations in tropical and subtropical regions of Asia (Li et al. 2019b). Due to its perennial nature, widespread area of cultivation, and the complex, ecological environment of tea plantations, tea suffers from a number of diseases, largely