A study of the regional distribution of bone formed around hydroxyapatite implants in the tibiae of streptozotocin-induced diabetic rats using multiple fluorescent labeling and confocal laser scanning microscopy
A study of the regional distribution of bone formed around hydroxyapatite implants in the tibiae of streptozotocin-induced diabetic rats using multiple fluorescent labeling and confocal laser scanning microscopy
复制标题
DOI：
10.1902/jop.1997.68.12.1169
复制
发表时间：
1997-12-01
影响因子：
4.3
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Iyama, S;Takeshita, F;Tanaka, T
本研究的目的是比较正常（对照）大鼠与糖尿病（DM）动物在种植体植入前 2 周由链脲佐菌素诱导的羟基磷灰石（HA）种植体周围骨形成的数量和区域分布。分别于植入后第7、14和21天注射钙黄绿素（GAL）、茜素络合酮（AL）和四环素（TC），并于植入后第28天处死大鼠。然后制备两组HA-骨界面的70μm未脱钙切片用于共焦激光扫描显微镜(CLSM)观察。在两组中，骨形成从HA表面发展到骨内膜、骨膜或骨髓。在对照组中，靠近内膜和骨膜的HA周围，新骨显示出三种颜色层（GAL、AL和TC）的广泛分层图案，但在IDM组中，第21天TC的标记密度较低。相比之下，在HA表面的外侧部分（远离内膜和骨膜），对照组的骨形成明显较少，而在DM组中，骨形成几乎完全受到抑制。这些发现表明，对照组中HA周围的骨形成是从HA表面开始的，而在DM组中，沿着远离内膜和骨膜的HA外侧部分的骨形成几乎完全受到抑制。此外，还表明在靠近内膜和骨膜的沿着HA的新生骨中，仅第21天的钙化被抑制。
THE PRESENT STUDY WAS DESIGNED to compare the amount and regional distribution of bone formation around hydroxyapatite (HA) implants in normal (control) rats with that of animals with diabetes mellitus (DM), induced by streptozotocin 2 weeks prior to implant placement. Calcein (GAL), alizarin complexone (AL), and tetracycline (TC) were injected on the 7th, 14th, and 21st days after implantation, respectively, and the rats were sacrificed on the 28th day after implantation. Seventy-mu m undecalcified sections of the HA-bone interface in both groups were then prepared for confocal laser scanning microscopy (CLSM) observation. In both groups, bone formation developed from the HA surface to the endosteum, periosteum, or bone marrow. In the control group, around the HA close to the endosteum and periosteum, the new bone showed an extensive lamination pattern of three color layers (GAL, AL, and TC), but in the IDM group the labeling density of TC on the 21st day was low. In contrast, on the lateral part of the HA surface (away from the endosteum and periosteum), there was considerably less bone formation in the control group, and in the DM group it was almost completely suppressed. These findings indicate that bone formation around the HA was initiated from the HA surface in the control group, while in the DM group, bone formation along the lateral part of the HA away from the endosteum and periosteum was almost completely suppressed. Furthermore, it is also suggested that in the new bone along the HA close to the endosteum and periosteum, only calcification on the 21st day was depressed.