An apple MYB transcription factor regulates cold tolerance and anthocyanin accumulation and undergoes MIEL1-mediated degradation
An apple MYB transcription factor regulates cold tolerance and anthocyanin accumulation and undergoes MIEL1-mediated degradation
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苹果 MYB 转录因子调节耐冷性和花青素积累并经历 MIEL1 介导的降解
DOI：
10.1111/pbi.13201
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发表时间：
2019-07-10
影响因子：
13.8
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
An, Jian-Ping;Wang, Xiao-Fei;Hao, Yu-Jin
MYB转录因子通过与碱性螺旋-环-螺旋(BHLH)转录因子的相互作用，在植物生长发育中发挥着不同的作用。MdBHLH33是一种苹果bHLHTf基因，通过激活MdCBF2和MdDFR的表达，在耐冷性和花色苷积累方面起到积极的调节作用。本研究还发现MYB Tf MdMYB308L对苹果的耐冷性和花青素的积累也有正向调节作用。我们发现MdMYB308L与MdBHLH33相互作用，并增强其与MdCBF2和MdDFR启动子的结合。此外，苹果环E3泛素连接酶MYB30-相互作用的E3连接酶1(MdMIEL1)是一种与MdMYB308L相互作用的蛋白，促进了MdMYB308L的泛素化降解，从而负调控苹果的耐冷性和花青素的积累。这些结果表明，MdMYB308L通过与MdbHLH33相互作用，对苹果的耐冷性和花青素积累起到积极的调节作用，并经历了MdMIEL1介导的蛋白质降解。MYB-bHLH蛋白复合体的动态变化似乎在植物生长发育的调控中起着关键作用。
MYB transcription factors (TFs) have been demonstrated to play diverse roles in plant growth and development through interaction with basic helix-loop-helix (bHLH) TFs. MdbHLH33, an apple bHLH TF, has been identified as a positive regulator in cold tolerance and anthocyanin accumulation by activating the expressions of MdCBF2 and MdDFR. In the present study, a MYB TF MdMYB308L was found to also positively regulate cold tolerance and anthocyanin accumulation in apple. We found that MdMYB308L interacted with MdbHLH33 and enhanced its binding to the promoters of MdCBF2 and MdDFR. In addition, an apple RING E3 ubiquitin ligase MYB30-INTERACTING E3 LIGASE 1 (MdMIEL1) was identified to be an MdMYB308L-interacting protein and promoted the ubiquitination degradation of MdMYB308L, thus negatively regulated cold tolerance and anthocyanin accumulation in apple. These results suggest that MdMYB308L acts as a positive regulator in cold tolerance and anthocyanin accumulation in apple by interacting with MdbHLH33 and undergoes MdMIEL1-mediated protein degradation. The dynamic change in MYB-bHLH protein complex seems to play a key role in the regulation of plant growth and development.