Effects of trichlorfon on maternal estrous cycle, oocyte maturation, and near-term fetal developmental outcome in mice.
Effects of trichlorfon on maternal estrous cycle, oocyte maturation, and near-term fetal developmental outcome in mice.
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DOI：
10.2486/indhealth.ms1275
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发表时间：
2011
影响因子：
2
通讯作者：
G. Ding;Shufang Zhou;Ying Tian;Yu Gao;R. Shi
中科院分区：
文献类型：
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作者：
G. Ding;Shufang Zhou;Ying Tian;Yu Gao;R. Shi
敌百虫（TCF）是一种广泛使用的广谱有机磷农药。很少有研究评估TCF在低水平暴露后（尤其是长期暴露后）对生殖毒性的影响。本研究评估了母亲通过饮用水连续30天暴露于2、10或50 mg/kg体重/d TCF后，TCF对雌性小鼠发情周期、卵母细胞成熟和近足月胎儿发育结局的直接影响。50 mg/kg/d TCF给药组中的雄性和雌性胎仔体重均显著降低;但2 mg/kg/d和10 mg/kg/d TCF给药组中未发生这种情况。TCF处理组与对照组在卵母细胞成熟率、第一极体排出率、动情周期等方面无显著差异。在TCF处理组中未观察到胎儿外部畸形的发生率增加。在10和50 mg/kg/d TCF给药组中，母体肝脏重量以剂量依赖性方式显著降低。卵巢、胸腺、肾、脾、肺、心脏或脑等器官的重量未发现显著变化。2 mg/kg/d和10 mg/kg/d TCF对任何体内生殖和发育终点均无影响，表明在暴露量低于10 mg/kg/d时不会发生TCF生殖毒性。
Trichlorfon (TCF) is a widely used broad-spectrum agricultural organophosphate (OP) pesticide. Few studies have evaluated the effects of TCF on reproductive toxicity after low-level exposure, especially after long-term exposure. This study assessed the direct effects of TCF on estrous cycle, oocyte maturation in female mice, and developmental outcome in near-term fetuses after 30 consecutive days of maternal exposure to 2, 10, or 50 mg/kg body weight/d TCF via drinking water. Both male and female fetuses in the 50 mg/kg/d TCF-treated group had significantly reduced body weights; but this did not occur in the 2 mg/kg/d and 10 mg/kg/d TCF-treated groups. No difference in oocyte maturation, including the percentages of germinal vesicle breakdown (GVBD) and first polar body (PB1) extrusion, or in estrous cycle was found between the control and TCF-treated groups. No increased incidence of fetal external malformations was observed in the TCF-treated groups. Significant decreases in maternal liver weights occurred in the 10 and 50 mg/kg/d TCF-treated groups in a dose-dependent manner. No significant changes were found in the weight of organs such as the ovaries, thymus, kidneys, spleen, lungs, heart or brain. The lack of effects of 2 mg/kg/d and 10 mg/kg/d TCF on any in vivo reproductive and developmental endpoints examined suggest that no TCF reproductive toxicity occurs at exposures less than 10 mg/kg/d.