Genotyping and PCR Detection of Potential Virulence Genes in Campylobacter jejuni and Campylobacter coli Isolates from Different Sources in Poland
Genotyping and PCR Detection of Potential Virulence Genes in Campylobacter jejuni and Campylobacter coli Isolates from Different Sources in Poland
复制标题
DOI：
10.1007/s12223-010-0025-6
复制
发表时间：
2010-03-01
影响因子：
2.6
通讯作者：
Klimuszko, D.
中科院分区：
文献类型：
--
作者：
Krutkiewicz, A.;Klimuszko, D.
在来自儿童、鸡、猪和狗的波兰空肠弯曲菌和结肠弯曲菌分离株人群中，确定了潜在毒力标志物的流行率。74株C. jejuni和15 C. PCR检测大肠杆菌。在从儿童、鸡和狗获得的分离株中发现了5种不同的推定毒力和毒素基因（flaA、cdtA、cdtB、cdtC和cj0588）的高流行率。从猪中分离的菌株中这些基因的出现与从其他来源分离的菌株显着不同。弯曲杆菌基因分型的两种方法。采用flaA分型和ADSRRS指纹图谱法对100株弯曲杆菌进行了分型，并首次将ADSRRS指纹图谱法用于弯曲杆菌属的研究。菌株在从鸡和狗分离的菌株中以及从鸡和儿童分离的菌株中证明了遗传特征的相似性。从猪体内分离的两株C. jejuni和C.大肠埃希菌与其他来源的分离株不聚群。
The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was detected by PCR. High prevalence of five different putative virulence and toxin genes (flaA, cdtA, cdtB, cdtC, and cj0588) was found among isolates obtained from children, chickens and dogs. The occurrence of these genes among isolates obtained from pigs was significantly different than for strains isolated from other sources. Two methods for genotyping Campylobacter spp. strains were applied - flaA-typing, and ADSRRS-fingerprinting method, which was used for the first time for Campylobacter spp. strains. Similarity of the genetic profiles was demonstrated in strains isolated from chickens and dogs, and in isolates from chickens and children. Strains isolated from pigs, both C. jejuni as well as C. coli, did not group with isolates from other sources.