The three-dimensional structure of the Na,K-ATPase from electron microscopy.
The three-dimensional structure of the Na,K-ATPase from electron microscopy.
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DOI：
10.1083/jcb.105.1.1
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发表时间：
1987-07
期刊：
影响因子：
--
通讯作者：
Smith PR
中科院分区：
文献类型：
--
作者：
Mohraz M;Simpson MV;Smith PR
通过电镜和图像重建研究了Na， k - atp酶的结构。利用负染色二聚体薄片倾斜角度+/- 60度的样品数据，获得了该酶的三维结构，总分辨率为2.5 nm。重建显示了一个复杂的质量分布，包括从细胞膜细胞质侧延伸约6.0 nm的成对分子带。分子包膜由一个巨大的“体”组成，“叶”和“臂”结构从中伸出。体呈柱状，相对于膜平面倾斜。负责二聚体形成的相互作用区域位于两个体之间，在重建中清晰可见。它已被确定为α亚基氨基末端部分的一个片段。连接带的臂靠近膜，很可能是由β亚基形成的。
The structure of Na,K-ATPase has been studied by electron microscopy and image reconstruction. A three-dimensional structure of this enzyme has been obtained to an overall resolution of 2.5 nm using data from specimens of negatively stained dimer sheets tilted through a range of angles +/- 60 degrees. The reconstruction shows a complex mass distribution consisting of ribbons of paired molecules extending approximately 6.0 nm from the cytoplasmic side of the membrane. The molecular envelope consists of a massive "body" with "lobe" and "arm" structures projecting from it. The body has a columnar shape and is tilted with respect to the plane of the membrane. The region of interaction responsible for dimer formation is located between two bodies and is clearly visible in the reconstruction. It has been identified as a segment in the amino-terminal portion of the alpha subunit. The arms that interconnect the ribbons are located close to the membrane and are most probably formed by the beta subunits.