Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons.
Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons.
复制标题
DOI：
10.1002/jbm.a.37031
复制
发表时间：
2020-06
期刊：
影响因子：
--
通讯作者：
Haifeng Liu;Yuqing Wang;Yi Yang;Anqing Wang;Chongquan Huang;Zhijun Zhao;Ping Li;Meili Liu-Meili-Li
中科院分区：
文献类型：
--
作者：
Haifeng Liu;Yuqing Wang;Yi Yang;Anqing Wang;Chongquan Huang;Zhijun Zhao;Ping Li;Meili Liu-Meili-Li
石墨烯作为一种高导电材料，被引入到丝素蛋白（SF）基底中，有望用于制备用于神经组织工程的电活性柔性支架。众所周知，排列的形态可以促进细胞的粘附和定向生长。本研究的目的是通过静电纺丝技术制备用于神经组织工程的石墨烯/SF（G/SF）定向导电支架。支架的物理化学表征结果表明，随着石墨烯含量的增加（A0% G/SF、A1% G/SF、A2% G/SF、A3% G/SF），支架的力学性能和电化学性能不断提高，但当石墨烯含量达到4%（A4% G/SF组）时，支架的力学性能下降。体外细胞实验结果表明，所有定向G/SF支架材料对原代培养的脊髓神经元均无神经毒性。此外，与对照组相比，Netrin-1表达的上调显著增强了所有对齐组中的神经突伸长。因此，A3% G/SF支架不仅具有最佳的力学性能和电化学性能，而且具有促进神经突起生长的能力，有望成为神经再生或神经工程中电活性组织的理想支架材料。
Graphene, as a highly conducting material, incorporated into silk fibroin (SF) substrates is promising to fabricate an electroactive flexible scaffolds toward neural tissue engineering. It is well known that aligned morphology could promote cell adhesion and directional growth. The purpose of this study was to develop aligned conductive scaffolds made of graphene and SF (G/SF) by electrospinning technique for neural tissue engineering applications. The physicochemical characterization of scaffolds revealed that the mechanical and electrochemical property of aligned G/SF scaffolds continually raised with the increasing contents of graphene (A0% G/SF, A1% G/SF, A2% G/SF, A3% G/SF), but the mechanical property descended when the graphene concentration reached to 4% (the A4% G/SF group). The results of cell experiment in vitro indicated that all the aligned G/SF scaffolds were no neurotoxic to primary cultured spinal cord neurons. In addition, the neurite elongation in all aligned groups was significantly enhanced by the up-regulation of Netrin-1 expression compared to them in the control group. Thus, A3% G/SF scaffolds not only possessed the optimal property based on the mechanical and electrochemical performances, but also displayed beneficial capability to neurite outgrowth, which might perform a suitable candidate to successfully scaffold electrically active tissues during neural regeneration or engineering.