PRODUCTION OF A MUTATION IN MOUSE EN-2 GENE BY HOMOLOGOUS RECOMBINATION IN EMBRYONIC STEM-CELLS
PRODUCTION OF A MUTATION IN MOUSE EN-2 GENE BY HOMOLOGOUS RECOMBINATION IN EMBRYONIC STEM-CELLS
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DOI：
10.1038/338153a0
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发表时间：
1989-03-09
期刊：
影响因子：
64.8
通讯作者：
ROSSANT, J
中科院分区：
文献类型：
--
作者：
JOYNER, AL;SKARNES, WC;ROSSANT, J
只有结合分子和突变分析，才能对控制发育事件的基因的功能有全面的了解。一个假定的发育基因是小鼠enrailed-like基因En-2，它是凭借其广泛的同源性分离到果蝇enrailed，这有助于在发展中的昆虫1节的控制。我们的原位杂交分析表明，En-2的表达仅限于发育中的中枢神经系统的一个特定的域从8天的发展2，表明在建立空间域在大脑中的基因的作用。不幸的是，没有En-2突变可用于进一步阐明其在发育中的功能。为此，我们在这里报告的三个多能胚胎干细胞系的分离，其中一个拷贝的homoeobox含有基因，En-2，已被改变的同源重组。
A FULL understanding of the function of genes that control developmental events can be obtained only by a combination of molecular and mutational analysis. One putative developmental gene is the mouse engrailed-like geneEn-2, which was isolated by virtue of its extensive homology toDrosophila engrailed, which contributes to the control of segmentation in the developing insect1. Our hybridization analysisin situhas revealed that expression ofEn-2is restricted to a specific domain of the developing central nervous system from 8 days of development on2, indicating a role for the gene in establishing spatial domains in the brain. Unfortu-nately noEn-2mutations are available to elucidate further its function in development. To this end, we report here the isolation of three pluripotent embryonic stem cell lines in which one copy of the homoeobox-containing gene,En-2, has been altered by homologous recombination.