Circular configuration of the genome of ascoviruses
Circular configuration of the genome of ascoviruses
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DOI：
10.1099/0022-1317-80-6-1537
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发表时间：
1999-06-01
影响因子：
3.8
通讯作者：
Brown, TM
中科院分区：
文献类型：
--
作者：
Cheng, XW;Carner, GR;Brown, TM
应用限制性内切酶（REN）消化、常规凝胶电泳、脉冲场凝胶电泳和Southern杂交分析，从夜蛾科（Noctuidae）两种昆虫[草地贪夜蛾（Spodoptera frugiperda）和棉铃虫（Helicoverpa zea）]中分离到的囊膜病毒（Ascovirus）的基因组DNA中观察到一个环状构型。基于线性和环状DNA在单和双REN消化后在琼脂糖凝胶电泳上解析的片段数目的差异，建立了囊病毒基因组DNA的这种环状构型。纯化后的病毒基因组DNA被剪切。
A circular configuration of genomic DNA was observed in ascoviruses isolated from two species of insects of the family Noctuidae [fall armyworm (Spodoptera frugiperda) and cotton bollworm (Helicoverpa zea)] using restriction endonuclease (REN) digestion, conventional gel electrophoresis, pulsed-field gel electrophoresis and Southern hybridization analysis. This circular configuration of ascovirus genomic DNA was established based on the difference between linear and circular DNA in the numbers of fragments resolved on agarose gel electrophoresis after single and double REN digestion. Genomic DNA of ascoviruses was found to be sheared after purification.