Expression of platelet-derived growth factor (PDGF)-A, PDGF-B and the PDGF-alpha receptor, but not the PDGF-beta receptor, in human malignant melanoma in vivo
Expression of platelet-derived growth factor (PDGF)-A, PDGF-B and the PDGF-alpha receptor, but not the PDGF-beta receptor, in human malignant melanoma in vivo
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DOI：
10.1046/j.1365-2133.1996.d01-1092.x
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发表时间：
1996-12-01
影响因子：
10.3
通讯作者：
Antoniades, HN
中科院分区：
文献类型：
--
作者：
Barnhill, RL;Xiao, M;Antoniades, HN
生长因子在皮肤恶性黑色素瘤（CMM）的发生和发展中的潜在作用引起了人们极大的兴趣。已证明血小板源性生长因子（PDGF）由黑色素瘤细胞系和体内转移性黑色素瘤分泌。据报道，PDGF还刺激肿瘤基质和新血管的发展。我们通过免疫组化（IHC）和原位杂交（ISH）研究了原发性和转移性黑色素瘤以及正常皮肤标本中PDGF及其受体的表达。冰冻切片与S-35标记的核糖核酸探针和PDGF-AA、PDGF-alpha受体、PDGF-BB和PDGF-beta受体的抗体一起孵育。原发性和转移性黑色素瘤通过IHC和ISH均显示PDGF-AA、PDGF-BB和PDGF-alpha受体的显著表达，与正常皮肤中仅背景表达相比，我们没有观察到PDGF-beta受体在黑色素瘤中的表达。我们的研究结果表明，PDGF可能作为一种自分泌生长因子，以及血管生成因子，在CMM肿瘤的发展。PDGF-alpha受体而不是β受体的这种表达在实体瘤中可能是独特的。
There has been considerable interest in the potential role of growth factors in the initiation and development of cutaneous malignant melanoma (CMM). Platelet-derived growth factor (PDGF) has been shown to be secreted by melanoma cell lines and by metastatic melanoma in vivo. PDGF also has been reported to stimulate the development of tumour stroma and new blood vessels. We studied the expression of PDGF and its receptors by both immunohistochemistry (IHC) and in situ hybridization (ISH) in primary and metastatic melanoma and in normal skin specimens. Cryostat sections were incubated with S-35-labelled riboprobes and antibodies for PDGF-AA, PDGF-alpha receptor, PDGF-BB and PDGF-beta receptor, Both primary and metastatic melanoma exhibited significant expression of PDGF-AA, PDGF-BB and PDGF-alpha receptor by both IHC and ISH, compared with only background expression in normal skin, We did not observe expression of PDGF-beta receptor in melanoma. Our results suggest that PDGF may function as an autocrine growth factor, as well as an angiogenesis factor, in CMM tumour development. This expression of the PDGF-alpha receptor rather than the beta receptor may be unique among solid tumours.