The Yersinia high-pathogenicity island (HPI) carried by a new integrative and conjugative element (ICE) in a multidrug-resistant and hypervirulent Klebsiella pneumoniae strain SCsl1
The Yersinia high-pathogenicity island (HPI) carried by a new integrative and conjugative element (ICE) in a multidrug-resistant and hypervirulent Klebsiella pneumoniae strain SCsl1
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多重耐药和高毒力肺炎克雷伯菌菌株 SCsl1 中新的整合和接合元件 (ICE) 携带耶尔森氏菌高致病性岛 (HPI)
DOI：
10.1016/j.vetmic.2019.108481
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发表时间：
2019-12-01
影响因子：
3.3
通讯作者：
Zhang, Anyun
中科院分区：
文献类型：
--
作者：
Liu, Dan;Yang, Yongqiang;Zhang, Anyun
多重耐药和高毒力肺炎克雷伯菌（hvKP）对公共卫生构成重大风险。为了更好地了解多重耐药和高毒力克雷伯氏菌的分子特征。pneumoniae，15株K.从病猪的肝脏、血液和鸡粪中收集肺炎菌株。所有K。对肺炎分离株进行抗菌药物敏感性测试、串测试、多位点序列分型和全基因组测序。七千发现肺炎分离株携带mcr-1.1基因。其中，一株耐多药的高毒力K.发现从患病猪的肝脏分离的肺炎菌株SCS 11携带16个抗性基因（例如，mcr-1.1）和16个毒力基因。此外，一个新的整合和接合元件，命名为ICEKpSL 1，在SCsl 1中，它包含一个完整的耶尔森菌高致病性岛（HPI）。该元件可以从染色体上切除形成环状中间体，表明耶尔森氏菌致病岛的潜在传播。克雷伯氏菌多药耐药性和超强毒力的出现。动物肺炎需要进一步监测。
Multidrug-resistant and hypervirulent Klebsiella pneumoniae (hvKP) poses a significant risk to public health. To better understand the molecular characteristics of multidrug-resistant and hypervirulent K. pneumoniae of animal origin, fifteen K. pneumoniae strains from the liver, blood of sick pigs and chicken feces were collected. All K. pneumoniae isolates were subjected to antimicrobial susceptibility testing, string test, multi-locus sequence typing and whole genome sequencing. Seven K. pneumoniae isolates were found carrying the mcr-1.1 gene. Among them, a multidrug-resistant and hypervirulent K. pneumoniae strain SCsl1 isolated from the liver of a diseased pig was found to harbor 16 resistance genes (e.g., mcr-1.1) and 16 virulence genes including aerobactin. Moreover, a novel integrative and conjugative element, named ICEKpSL1, was identified in SCsl1, which contains a full Yersinia high-pathogenicity island (HPI). This element could be excised from the chromosome to form a circular intermediate, indicating potential transmission of the Yersinia pathogenicity island. The emergence of multidrug-resistance and hypervirulence in K. pneumoniae from animals warrants further surveillance.