Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7
Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7
复制标题
用于检测大肠杆菌 O157:H7 的侧流条传感器的图像分析
DOI：
10.5307/jbe.2013.38.4.335
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发表时间：
2013
影响因子：
0.8
通讯作者：
Changyeun Mo
中科院分区：
文献类型：
--
作者：
Giyoung Kim;Jihea Moon;Saetbyeol Park;Younchang Jang;Jongguk Lim;Changyeun Mo
目的：研制一种用于检测不同样品中致病性大肠埃希菌O157：H7的侧向流动纸条传感器。此外，还评估了使用图像分析方法改善条带传感器解释的可行性。方法：利用硝酸纤维素侧向流膜制作侧向流条传感器。分别以胶体金和大肠杆菌O157：H7抗体作为标记和受体。分别以硝酸纤维素膜上手工斑点的O157：H7抗体和抗鼠抗体为试验点和对照点。用PBS或食品样品中连续稀释的大肠杆菌O157：H7细胞对横向流动纸条传感器检测O157：H7的可行性进行了评价。用图像分析的方法对横向流条传感器的测试结果进行了测量。结果：随着细胞浓度的升高，受试点的强度开始增加。该传感器对PBS和鸡肉提取物的检测灵敏度分别为CFU/mLO157：H7。结论：横向流动纸片传感器和图像分析方法可在20min内检测到O157：H7大肠杆菌，明显快于常规平板计数法。
Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.