Application of 99mTc labeled chimeric Fab fragments of monoclonal antibody A7 for immunoscintigraphy of pancreatic carcinoma
Application of 99mTc labeled chimeric Fab fragments of monoclonal antibody A7 for immunoscintigraphy of pancreatic carcinoma
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DOI：
10.1002/jso.10311
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发表时间：
2003-11-01
影响因子：
2.5
通讯作者：
Yamagishi, H
中科院分区：
文献类型：
--
作者：
Otsuji, E;Matsumura, H;Yamagishi, H
背景资料：胰腺癌预后差的原因之一是胰腺癌的早期诊断困难。一种可能性是使用放射性标记的单克隆抗体（Mab）进行放射免疫闪烁成像。用Tc-99 m标记Mab A7（人/小鼠）Fab片段（chA 7 Fab），并检查Tc-99 m标记的chA 7 Fab在荷人胰腺癌裸鼠中的分布。注射后2 ~ 6 h，Tc-99 m-chA 7 Fab的肿瘤蓄积量大于Tc-99 m-A7。Tc-99 m标记的chA 7 Fab在血液中的消失比Tc-99 m标记的AT快。在除肾脏外的所有切除的正常组织中，Tc-99 m标记的chA 7 Fab的蓄积量低，并且与Tc-99 m标记的A7的蓄积量相似。由于Tc-99 m的半衰期短（6小时），在肿瘤中快速积累的chA 7 Fab可能是比完整Mab A7更好的Tc-99 m载体。(C)2003 Wiley-Liss，Inc.
Background: One of the reasons for the poor prognosis of pancreatic carcinoma is the difficulty of obtaining an early diagnosis of pancreatic carcinoma. One possibility is the application of radioimmunoscintigraphy using radiolabeled monoclonal antibodies (Mabs).Methods: We labeled chimeric (human/mouse) Fab fragments of Mab A7 (chA7Fab) with Tc-99m and examined the distribution of Tc-99m-labeled chA7Fab in nude mice bearing human pancreatic carcinoma.Results: The tumor accumulation of Tc-99m-labeled chA7Fab was larger than that of Tc-99m-labeled A7 from 2 to 6 hr after injection. Tc-99m-labeled chA7Fab disappeared from blood more rapidly than Tc-99m-labeled AT For all resected normal tissues except kidney, the accumulation of Tc-99m-labeled chA7Fab was low and similar to that of Tc-99m-labeled A7.Conclusions: Because the half-life of Tc-99m is short (6 hr), chA7Fab, which accumulates rapidly in tumors, may be a better carrier of Tc-99m than intact Mab A7. (C) 2003 Wiley-Liss, Inc.