VECTORS FOR DROSOPHILA P-ELEMENT-MEDIATED TRANSFORMATION AND TISSUE-CULTURE TRANSFECTION
VECTORS FOR DROSOPHILA P-ELEMENT-MEDIATED TRANSFORMATION AND TISSUE-CULTURE TRANSFECTION
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DOI：
10.1016/0378-1119(88)90177-1
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发表时间：
1988-12-30
期刊：
影响因子：
3.5
通讯作者：
LIPSHITZ, HD
中科院分区：
文献类型：
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作者：
THUMMEL, CS;BOULET, AM;LIPSHITZ, HD
我们描述了9个可用于研究果蝇基因调控和功能的p元素载体。这些载体被设计用于种系转化和细胞培养转染试验。其中一组包含5个可用于研究转录调控序列的P元素。这些载体包含几个独特的限制性位点，用于插入cat或lacZ报告基因上游的外源启动子。两个。-半乳糖苷酶编码载体也需要插入起始密码子来翻译报告酶，因此可以用于研究翻译调控序列。第二组P元件由包含果蝇细胞质肌动蛋白5C启动子和聚腺苷化信号的四个载体组成。在插入外源DNA片段后，这些载体直接表达编码的RNA和蛋白质。
We describe nine P-element vectors that can be used to study gene regulation and function in Drosophila. These vectors were designed for use in germline transformation and cell culture transfection assays. One set consists of five P elements that can be used to study transcriptional regulatory sequences. These vectors contain several unique restriction sites for insertion of a foreign promoter upstream for either a cat or lacZ reporter gene. Two of the .beta.-galactosidase-coding vectors also require the insertion of a start codon for translation of the reporter enzyme and thus can be used to study translational regulatory sequences. The second set of P elements consists of four vectors that contain the Drosophila cytoplasmic actin 5C promoter and polyadenylation signals. Upon insertion of a foreign DNA segment, these vectors direct constitutive expression of the encoded RNA and protein.