EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN PROXIMAL TUBULES OF MOUSE KIDNEY - ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE
EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN PROXIMAL TUBULES OF MOUSE KIDNEY - ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE
复制标题
DOI：
10.1177/14.4.291
复制
发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
KARNOVSKY, MJ
中科院分区：
文献类型：
--
作者：
GRAHAM, RC;KARNOVSKY, MJ
采用新的超微结构细胞化学技术研究了静脉注射辣根过氧化物酶在小鼠肾近曲小管中吸收的早期阶段。在注射后 90 秒处死的动物中，在刷状缘膜和顶管想象中发现了反应产物。它从后一个结构被运输到顶端液泡，在其中逐渐浓缩形成蛋白质吸收液滴。该方法采用 3,3[image]-dlaminobenzidine 作为可氧化底物，定位清晰且灵敏。该系统有利于研究肾小管蛋白质吸收的早期阶段，因为可以轻松检测到膜上以及肾小管和囊泡中的少量蛋白质。该方法对于研究多种其他细胞和组织中的蛋白质转运似乎也很有前景。
The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studies with a new ultra-structural cytochemical technique. In animals killed as early as 90 sec after injection, reaction product was found on the brushborder membranes and in the apical tubular imaginations. From the latter structures it was transported to the apical vacuoles, in which it was progressively concentrated to form protein absorption droplets. The method, which employs 3,3[image]-dlaminobenzidine as oxidizable substrate, gives sharp localization and is sensitive. This system is advantageous in studying the early stages of renal tubular protein absorption, since small amounts of protein on membranes and in tubules and vesicles can be detected easily. The method also appears promising for studying protein transport in a variety of other cells and tissues.