Nucleic aptamer modified porous reduced graphene oxide/MoS2 based electrodes for viral detection: Application to human papillomavirus (HPV)
Nucleic aptamer modified porous reduced graphene oxide/MoS2 based electrodes for viral detection: Application to human papillomavirus (HPV)
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DOI：
10.1016/j.snb.2018.02.065
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发表时间：
2018-06-01
影响因子：
8.4
通讯作者：
Szunerits, Sabine
中科院分区：
文献类型：
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作者：
Chekin, Fereshteh;Bagga, Komal;Szunerits, Sabine
仅次于石墨烯纳米材料，二硫化钼 (MoS2) 具有较大的表面积，可以增强其生物传感性能。在这项工作中，我们研究了连续用多孔还原氧化石墨烯（prGO）和硫化钼（MoS2）修饰的玻璃碳（GC）电极的性能，用于灵敏和选择性检测人乳头瘤病毒（HPV）的L1主要衣壳蛋白。由于高效进行血清学检测和 HPV 培养的困难，基于分子识别的工具变得非常重要。我们在这里开发了一种用于 HPV 的电化学传感器，其电极与适体 Sc5-c3 共价功能化，Sc5-c3 是一种针对 HPV-16 L1 蛋白的 RNA 适体。利用差分脉冲伏安法（DPV）和优化的传感器接口，[Fe(CN)6]4-等氧化还原对的峰值电流密度与HPV-16 L1蛋白浓度在0.2-2 ng mL(-1) (3.5 pM-35.3 pM)范围内呈线性关系，检测限为0.1 ng mL(-1) (1.75 pM)。交叉反应性研究表明，其对 HPV-16 E6 等潜在干扰物质具有高选择性，为护理点设备的开发概念开辟了新的机遇。 (C) 2018 Elsevier B.V. 保留所有权利。
Next to graphene nanomaterials, molybdenum disulfide (MoS2) offers large surface area that can enhance its biosensing performance. In this work, we investigate the performance of glassy carbon (GC) electrodes modified successively with porous reduced graphene oxide (prGO) and molybdenum sulfide (MoS2) for the sensitive and selective detection of the L1-major capsid protein of human papilloma virus (HPV). Owing to the difficulties to perform serological assays and HPV culture efficiently, tools based on molecular recognition are becoming of great importance. We developed here an electrochemical sensor for HPV upon covalent functionalization of the electrode with an aptamer Sc5-c3, a RNA aptamer targeted against the HPV-16 L1 protein. Using differential pulse voltammetry (DPV) and an optimized sensor interface, a linear relationship between the peak current density of a redox couple such as [Fe(CN)6]4- and the concentration of HPV-16 L1 proteins in the range of 0.2-2 ng mL(-1) (3.5 pM-35.3 pM) could be reached with a detection limit of 0.1 ng mL(-1) (1.75 pM). Cross-reactivity studies demonstrated high selectivity over potential interfering species such as HPV-16 E6, opening new opportunities of the developed concept for the development of point of care devices. (C) 2018 Elsevier B.V. All rights reserved.