Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding
Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding
复制标题
DOI：
10.1104/pp.17.00858
复制
发表时间：
2018-04-01
期刊：
影响因子：
7.4
通讯作者：
Reuscher, Stefan
中科院分区：
文献类型：
--
作者：
Minami, Anzu;Yano, Kenji;Reuscher, Stefan
水淹是限制植物生长和存活的环境因素。深水水稻（Oryza sativa）通过迅速伸长其节间来适应淹没，从而使其叶片保持在水面以上。我们进行了比较RNA测序转录组分析的拍摄基地区，包括基节，节间，和苗尖的两个发展阶段，从两个品种的对比深水生长反应。深水水稻品种C9285和非深水水稻品种台中65之间的转录组学比较揭示了两种基因型在淹水期间的相似和差异表达模式。与赤霉素生物合成、海藻糖生物合成、厌氧发酵、细胞壁修饰和包括乙烯响应因子在内的转录因子相关的基因的表达在品种之间存在显著差异。有趣的是，在这两个品种，茉莉酸含量下降，在地上部的淹没过程中，而外源茉莉酸抑制淹没诱导的节间伸长品种C9285，表明茉莉酸发挥作用的淹没响应。此外，一个有针对性的从头转录本组装揭示了特定于C9285的转录本，包括淹没诱导的生物胁迫相关基因。我们的多方面的转录组的方法，使用水稻芽基地区说明了深水和非深水水稻之间的淹没的差异反应。茉莉酸代谢似乎参与了深水水稻的淹水介导的节间伸长反应。
Water submergence is an environmental factor that limits plant growth and survival. Deepwater rice (Oryza sativa) adapts to submergence by rapidly elongating its internodes and thereby maintaining its leaves above the water surface. We performed a comparative RNA sequencing transcriptome analysis of the shoot base region, including basal nodes, internodes, and shoot apices of seedlings at two developmental stages from two varieties with contrasting deepwater growth responses. A transcriptomic comparison between deepwater rice cv C9285 and nondeepwater rice cv Taichung 65 revealed both similar and differential expression patterns between the two genotypes during submergence. The expression of genes related to gibberellin biosynthesis, trehalose biosynthesis, anaerobic fermentation, cell wall modification, and transcription factors that include ethylene-responsive factors was significantly different between the varieties. Interestingly, in both varieties, the jasmonic acid content at the shoot base decreased during submergence, while exogenous jasmonic acid inhibited submergence-induced internode elongation in cv C9285, suggesting that jasmonic acid plays a role in the submergence response of rice. Furthermore, a targeted de novo transcript assembly revealed transcripts that were specific to cv C9285, including submergence-induced biotic stress-related genes. Our multifaceted transcriptome approach using the rice shoot base region illustrates a differential response to submergence between deepwater and nondeepwater rice. Jasmonic acid metabolism appears to participate in the submergence-mediated internode elongation response of deepwater rice.