Adhesion, shape, proliferation, and gene expression of mouse Leydig cells are influenced by extracellular matrix in vitro.
Adhesion, shape, proliferation, and gene expression of mouse Leydig cells are influenced by extracellular matrix in vitro.
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DOI：
10.1095/biolreprod44.1.157
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发表时间：
1991
影响因子：
3.6
通讯作者：
R. Vernon;T. Lane;J. Angello;H. Sage
中科院分区：
文献类型：
--
作者：
R. Vernon;T. Lane;J. Angello;H. Sage
哺乳动物睾丸间质室中间质细胞与细胞外基质（ECM）之间的相互作用尚未被表征。我们通过在不同的ECM基质上培养成鼠间质细胞，研究了ECM对成鼠间质细胞的影响。间质细胞粘附在I型胶原的水合凝胶（包括补充了IV、V或VIII型胶原的凝胶）或I、V或VIII型胶原的风干膜上的能力较弱。相反，细胞牢固地附着在纯化的IV型胶原、纤维连接蛋白或层粘连蛋白的底物上。间质细胞也能快速附着并强烈粘附在来源于小鼠恩格尔布雷斯-霍尔姆-群肉瘤（Matrigel）的凝胶基底膜基质上。间质细胞呈球形，在厚（1.5毫米）的基质层上形成聚集体；然而，在薄层（0.1 mm）上，由细长细胞索连接的细胞团网络在48小时内形成。在补充了I型胶原的厚层基质上也形成了类似的网络。在胶原I与基质比例高的基质上或未经处理的组织培养塑料上，间质细胞变平且不聚集。在诱导圆形的底物上，增殖受到抑制，细胞维持类固醇生成酶3 β -羟基类固醇脱氢酶长达2周。在间质细胞变平的条件下，它们分裂并停止表达这种酶。增殖的间质细胞也表现出SPARC（分泌蛋白，酸性且富含半胱氨酸）mRNA水平升高，SPARC是一种Ca2（+）结合的糖蛋白，与伴随形态发生和组织重塑的细胞形状变化有关。我们的研究结果表明，通过改变细胞外基质的组成，可以显著影响间质细胞的形状、关联、增殖和基因产物的表达。
Interactions between Leydig cells and the extracellular matrix (ECM) within the interstitial compartment of the mammalian testis have not been characterized. We have examined the influence of ECM on adult mouse Leydig cells by culturing the cells on different ECM substrates. Leydig cells adhere weakly to hydrated gels of type I collagen (including those supplemented with collagen types IV, V, or VIII), or to air-dried films of collagen types I, V, or VIII. In contrast, the cells attach firmly to substrates of purified type IV collagen, fibronectin, or laminin. Leydig cells also attach rapidly and adhere strongly to gelled basement membrane matrix derived from the murine Englebreth-Holm-Swarm sarcoma (Matrigel). Leydig cells assume spherical shapes and form aggregates on thick (1.5-mm) layers of Matrigel; however, on thin (0.1-mm) layers, networks of cell clusters linked by cords of elongated cells are formed within 48 h. Similar networks are formed on thick layers of Matrigel that are supplemented with type I collagen. On substrates with high ratios of collagen I to Matrigel or on untreated tissue culture plastic, Leydig cells flatten and do not aggregate. On substrates that induce rounded shapes, proliferation is inhibited and the cells maintain the steroidogenic enzyme 3 beta-hydroxysteroid dehydrogenase for as long as 2 wk. Under conditions where Leydig cells are flattened, they divide and cease expressing the enzyme. Proliferating Leydig cells also exhibit elevated levels of mRNA for SPARC (Secreted Protein, Acidic and Rich in Cysteine), a Ca2(+)-binding glycoprotein associated with changes in cell shape that accompany morphogenesis and tissue remodeling. Our results indicate that the shape, association, proliferation, and expression of gene products by Leydig cells can be significantly affected in vitro by altering the composition of the extracellular substratum.