Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis.
Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis.
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龙须菜多糖的表征和潜在抗肿瘤活性
DOI：
10.3390/md15040100
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发表时间：
2017-03-29
期刊：
影响因子：
5.4
通讯作者：
Xu N
中科院分区：
文献类型：
--
作者：
Kang Y;Wang ZJ;Xie D;Sun X;Yang W;Zhao X;Xu N
在许多海藻中已经发现了具有抗肿瘤特性的物质，其中包括一种可食用的薄叶藓多糖（PGL）。我们之前报道的转录组分析数据显示，PGL诱导的转录改变产生抗肺癌活性。为了确定PGL是如何对肿瘤有害的，我们纯化了PGL，表征了它的化学成分、分子量、糖和蛋白质含量，并研究了它的抗肿瘤活性。我们证明了PGL通过调节人肺癌细胞系A549、胃癌细胞系MKN28和小鼠黑色素瘤细胞系B16的细胞活力、形态、凋亡和凋亡相关的Fas/FasL信号通路发挥其抗肿瘤活性。我们的数据首次证明了PGL通过诱导凋亡抑制细胞增殖，而凋亡主要是由Fas/FasL介导的，这表明PGL可能是一种新的抗癌药物。
Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine alga Gracilariopsis lemaneiformis (PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antitumor activity. We demonstrated that PGL exerted its antitumor activities by modulating cell viability, morphology, apoptosis, and the apoptosis-related Fas/FasL signaling pathway in the human lung cancer cell line A549, the gastric cancer cell line MKN28, and the mouse melanoma cell line B16. Our data provide the first evidence that PGL inhibits cell proliferation by inducing apoptosis, which is largely mediated by Fas/FasL in cancer cells, suggesting that PGL might be a novel therapeutic agent against cancer.