Characterization of a Novel Plasmid-Borne Thiopeptide Gene Cluster in Staphylococcus epidermidis Strain 115
Characterization of a Novel Plasmid-Borne Thiopeptide Gene Cluster in Staphylococcus epidermidis Strain 115
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DOI：
10.1128/jb.02243-14
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Griffitts, Joel S.
中科院分区：
文献类型：
--
作者：
Bennallack, Philip R.;Burt, Scott R.;Griffitts, Joel S.
硫肽是细菌来源的经过高度修饰的小肽（12 至 17 个氨基酸）。该抗生素家族有 100 多个已知成员，其特征是在大环肽结构中存在含硫杂环和脱水残基。近年来，包括微球菌素 P1 在内的硫肽因其对细菌、真菌甚至原生动物具有强大的抗菌活性而受到广泛关注。已知微球菌素 P1 以核糖体为目标；然而，与其他硫肽一样，其生物合成和自身免疫机制尚不清楚。我们发现了表皮葡萄球菌的分离株，其 24 kb 质粒上含有硫肽生产和自我保护的基因。在这里，我们报告了该质粒的特征，鉴定了其编码的抗菌肽，并提供了靶点替换介导的自身免疫机制的证据。
Thiopeptides are small (12- to 17-amino-acid), heavily modified peptides of bacterial origin. This antibiotic family, with more than 100 known members, is characterized by the presence of sulfur-containing heterocyclic rings and dehydrated residues within a macrocyclic peptide structure. Thiopeptides, including micrococcin P1, have garnered significant attention in recent years for their potent antimicrobial activity against bacteria, fungi, and even protozoa. Micrococcin P1 is known to target the ribosome; however, like those of other thiopeptides, its biosynthesis and mechanisms of self-immunity are poorly characterized. We have discovered an isolate of Staphylococcus epidermidis harboring the genes for thiopeptide production and self-protection on a 24-kb plasmid. Here we report the characterization of this plasmid, identify the antimicrobial peptide that it encodes, and provide evidence of a target replacement-mediated mechanism of self-immunity.