Immunolocalization of two types of allatostatins in the central nervous system of the cricket Gryllus bimaculatus (Ensifera: Gryllidae)
Immunolocalization of two types of allatostatins in the central nervous system of the cricket Gryllus bimaculatus (Ensifera: Gryllidae)
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两种类型的尿抑素在蟋蟀 Gryllus bimaculatus（Ensifera：Gryllidae）中枢神经系统中的免疫定位
DOI：
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发表时间：
2013
影响因子：
1.3
通讯作者：
K. Hoffmann
中科院分区：
文献类型：
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作者：
Günther Witek;P. Verhaert;M. Lorenz;K. Hoffmann
用抗双斑蟋蟀AllatostatinA 1（Grb-ASTA 1）和AllatostatinB 1（Grb-ASTB 1）的抗肽抗血清（抗A和抗B），对蟋蟀脑、食管下神经节、心体（CC）和咽侧体（CA）的全系列切片进行过氧化物酶-抗过氧化物酶（PAP）免疫组织化学染色。双斑的这两种抗血清产生了大量的细胞和神经纤维的强烈染色。用抗A和抗B交替染色的连续切片证实，抗B通常染色更多的细胞和神经。广泛的免疫反应性在大脑后复合体表明，CC和CA可能代表的存储和/或释放网站的allatostatin类型或allatostatin样分子在大脑中产生的。
Two anti-peptide antisera (anti-A and anti-B) raised against Gryllus bimaculatus allatostatin A1 (Grb-AST A1) and B1 (Grb-AST B1), respectively, were applied in the peroxidase-antiperoxidase (PAP) immunohistochemical technique on complete series of sections from the brain, suboesophageal ganglion, corpora cardiaca (CC), and corpora allata (CA) of G. bimaculatus. Both antisera yielded intense staining of numerous cells and nerve fibres. Serial sections, alternately stained with anti-A and anti-B confirmed that the anti-B generally stained more cells and nerves. Extensive immunoreactivity in the retrocerebral complex suggests that the CC and CA may represent a storage and/or release site for both allatostatin types or allatostatin-like molecules produced in the brain.