Impact of Azospirillum sp. B510 on the Rhizosphere Microbiome of Rice under Field Conditions
Impact of Azospirillum sp. B510 on the Rhizosphere Microbiome of Rice under Field Conditions
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DOI：
10.3390/agronomy12061367
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发表时间：
2022-06
期刊：
影响因子：
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通讯作者：
M. Yasuda;K. Dastogeer;Elsie Sarkodee-Addo;Chihiro Tokiwa;Tsuyoshi Isawa;Satoshi Shinozaki;Shin Okazaki
中科院分区：
文献类型：
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作者：
M. Yasuda;K. Dastogeer;Elsie Sarkodee-Addo;Chihiro Tokiwa;Tsuyoshi Isawa;Satoshi Shinozaki;Shin Okazaki
生物肥料对作物相关微生物群落组成的影响越来越受到人们的关注。研究了固氮螺菌对细菌生长的影响。利用两个采样阶段(水稻营养生长期和收获期)的扩增序列分析了具有固氮能力的细菌菌株B510对水稻根际细菌和真菌群落结构的影响。主坐标分析(PCoA)表明，在营养生长期接种B510后，细菌群落发生了明显的变化，这与化学氮肥的接种效果非常相似。这一结果表明，在低氮条件下，接种B510对寄主植物的氮素吸收有很大的影响。最小判别分析(LDA)表明，接种B510显著增加了固氮梭菌、气单胞菌和芽孢杆菌的数量。相比之下，B510对真菌群落结构没有明显的影响。通过PICRUSt2对细菌的功能性质进行了鉴定，提示B510的氨基酸、糖和维生素的产生可能与B510的接种有关。我们的结果表明，接种B510通过在没有氮肥的情况下招募其他固氮细菌来影响细菌群落结构。
There has been increasing attention toward the influence of biofertilizers on the composition of microbial communities associated with crop plants. We investigated the impact of Azospirillum sp. B510, a bacterial strain with nitrogen-fixing ability, on the structure of bacterial and fungal communities within rice plant rhizospheres by amplicon sequencing at two sampling stages (the vegetative and harvest stages of rice). Principal coordinate analysis (PCoA) demonstrated a significant community shift in the bacterial microbiome when the plants were inoculated with B510 at the vegetative stage, which was very similar to the effect of chemical N-fertilizer application. This result suggested that the inoculation with B510 strongly influenced nitrogen uptake by the host plants under low nitrogen conditions. Least discriminant analysis (LDA) showed that the B510 inoculation significantly increased the N2-fixing Clostridium, Aeromonas and Bacillus populations. In contrast, there was no apparent influence of B510 on the fungal community structure. The putative functional properties of bacteria were identified through PICRUSt2, and this hinted that amino acid, sugar and vitamin production might be related to B510 inoculation. Our results indicate that B510 inoculation influenced the bacterial community structure by recruiting other N2-fixing bacteria in the absence of nitrogen fertilizer.