Efficient plant regeneration from embryogenic suspension cultures of sweetpotato
Efficient plant regeneration from embryogenic suspension cultures of sweetpotato
复制标题
DOI：
10.1007/s11627-001-0098-7
复制
发表时间：
2001-09-01
影响因子：
2.6
通讯作者：
Zhang, DP
中科院分区：
文献类型：
--
作者：
Liu, QC;Zhai, H;Zhang, DP
以15个中国甘薯品种和日本甘薯品种为试材，对甘薯（lpomoea batatas（L.）拉姆，我们成功地从胚性悬浮培养物中开发了有效的植物再生系统。从15个品种的茎尖获得的胚性愈伤组织，在含有9.05 μ M 2，4-二氯苯氧乙酸（2，4-D）的MS培养基中诱导胚性悬浮培养。建立了增殖快、分散性好的胚性悬浮培养体系。将来自胚发生悬浮培养物的大小为0.7-1.1分钟的细胞聚集体转移至。添加9-05 μ M 2，4-D的固体MS培养基，并形成具有体细胞胚的胚性愈伤组织。将具有体细胞胚的胚性愈伤组织进一步转移到补充有3.78 μ M脱落酸的MS培养基中，导致体细胞胚的萌发。在诱导后20周内，15个供试品种的细胞聚集体形成植株的频率达到约100%。这些小植株，当转移到土壤中时，显示100%存活。未观察到形态学变化。
Using 15, Chinese and Japanese cultivars of sweetpotato, lpomoea batatas (L.) Lam., we succeeded in developing an efficient plant regeneration system from embryogenic suspension cultures. The embryogenic callus derived from shoot apices of the 15 cultivars, was used to initiate embryogenic suspension cultures in Murashige and Skoog (MS) medium containing 9.05 muM 2,4-dichlorophenoxyacetic acid (2,4-D). Rapidly proliferating and well-dispersed embryogenic suspension cultures were established. Cell aggregates 0.7-1.1 min in size from embryogenic suspension cultures were transferred to. solid MS medium supplemented with 9-05 muM of 2,4-D and formed embryogenic, callus with somatic embryos. The embryogenic callus with somatic embryos was further transferred to MS medium supplemented with 3.78 muM of abscisic acid, resulting in the germination of somatic embryos. Within 20 wk after the initiation, the frequencies of cell aggregates forming plantlets reached approximately 100% for the 15 tested cultivars. These plantlets, when transferred to, soil, showed 100% survival. No morphological variations were observed.