Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome II: serological studies
Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome II: serological studies
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DOI：
10.3354/dao031209
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发表时间：
1997-12-30
影响因子：
1.4
通讯作者：
Olesen, NJ
中科院分区：
文献类型：
--
作者：
Lorenzen, E;Olesen, NJ
用ELISA和玻片凝集法分析了嗜冷黄杆菌分离株之间血清学区分的可能性。使用多克隆兔抗血清和全细胞制剂研究了25个丹麦菌株和20个来自其他欧洲国家的菌株。包括未吸收的以及吸收后的抗血清和来源于抗血清的纯化IG制剂。大多数分离物来源于虹鳟鱼苗综合征（RTFS）或冷水病（CWD）的临床暴发，但也有部分分离物来自无症状或其他不同症状的鱼类。ELISA结果显示，不同血清型的存在最为明显，但玻片凝集反应支持ELISA结果。在所研究的分离株中发现3种血清型：1种主要血清型（Th血清型）代表了大多数丹麦分离株和来自其他欧洲国家的分离株; 2种次要血清型（血清型艾德和Fp（T））也出现。血清型Th可进一步分为主要亚型Th-1和次要亚型Th-2。血清型Fp（T）由模式菌株F定义。psychrophilum NCIMB 1947（T），并且似乎主要包括来自无症状鱼或来自虹鳟鱼以外的鱼类的分离物。
The possibility of seroIogical differentiation between isolates of Flavobacterium psychrophilum was analyzed by ELISA and slide agglutination. Twenty-five Danish isolates and 20 isolates from other European countries were studied using polyclonal rabbit antisera and whole-cell preparations. Unabsorbed as well as reciprocally absorbed antisera and purified Ig preparations derived from the antisera were included. Most of the isolates originated from clinical outbreaks of rainbow trout fry syndrome (RTFS) or coldwater disease (CWD), but some were isolated from asymptomatic fish or from other fish species with different disease signs, The ELISA showed the existence of different serotypes most distinctly, but slide agglutination supported the ELISA results. Three serotypes were found among the isolates studied: 1 major serotype (serotype Th) represented most of the Danish isolates and isolates from other European countries; 2 minor serotypes (Serotypes Ed and Fp(T)) also occurred. Serotype Th could be further divided into a major subtype, Th-1, and a minor subtype, Th-2. Serotype Fp(T) was defined by the type strain F. psychrophilum NCIMB 1947(T), and seemed to include mostly isolates from asymptomatic fish or from fish species other than rainbow trout.