Pre-clinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep
Pre-clinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep
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绵羊组织中PrP蛋白检测羊痒病的临床前和临床诊断
DOI：
10.1136/vr.128.12.271
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Shinagawa
中科院分区：
文献类型：
--
作者：
Y. Ikegami;M. Ito;H. Isomura;E. Momotani;K. Sasaki;Y. Muramatsu;N. Ishiguro;M. Shinagawa
研究了检测绵羊淋巴网状器官中痒病相关纤维蛋白（PrP）作为诊断工具的有用性。通过蛋白质印迹分析，利用兔抗羊PrP多克隆抗体检测PrP。在表现出自然瘙痒病感染临床症状的六只羊中的五只的中枢神经系统 (CNS) 样本、六只羊中的四只的脾脏样本以及三只羊的淋巴结样本中检测到了 PrP。在六只临床和组织病理学异常的羊中的一只羊的脾脏和淋巴结样本中检测到 PrP，但在中枢神经系统样本中未检测到 PrP。该动物似乎处于疾病的早期临床阶段。对总共 47 只临床正常的绵羊进行了 PrP 检测。在 47 只羊中 3 只的脾脏样本和 39 只羊中 3 只的淋巴结样本中检测到了这种病毒。同样，在接种 14 个月后，从三只实验感染的绵羊中的一只通过手术获得的淋巴结样本中也检测到了 PrP。 PrP 阳性羊和剩下的一只 PrP 阴性羊分别在六个月和五个月后出现瘙痒病临床症状。实验感染18个月后被安乐死的一只羊的中枢神经系统、脾脏和淋巴结中的PrP呈阳性，但感染后2、8、16、18和21个月被宰杀或死亡的另外5只羊的PrP检测呈阴性或可疑。
The usefulness of detecting the scrapie-associated fibrillar protein (PrP) in the lymphoreticular organs of sheep as a diagnostic tool was investigated. The PrP was detected by means of a rabbit-anti-sheep PrP polyclonal antibody by Western blot analysis. PrP was detected in samples from the central nervous system (CNS) of five of six sheep showing clinical signs of natural scrapie infection, in spleen samples from four of the six sheep and in lymph node samples taken from three of the sheep. PrP was detected in the spleen and lymph node samples, but not in the CNS samples from one of the six sheep that was clinically and histopathologically abnormal. This animal appeared to be in the early clinical stage of the disease. A total of 47 clinically normal sheep were examined for the presence of PrP. It was detected in spleen samples from three of the 47 sheep and in lymph node samples from three of the 39 sheep tested. Similarly, PrP was detected in a sample of lymph node obtained surgically from one of three experimentally infected sheep 14 months after inoculation. The PrP-positive sheep and one of the remaining PrP-negative sheep showed clinical signs of scrapie six and five months later respectively. One sheep euthanased 18 months after experimental infection was positive for PrP in the CNS, spleen and lymph node, but five other sheep which were killed or died two, eight, 16, 18 and 21 months after infection were negative or doubtful for the detection of PrP.