Differences in the acidic sensitivity of transient receptor potential vanilloid 1 (TRPV1) between chickens and mice
Differences in the acidic sensitivity of transient receptor potential vanilloid 1 (TRPV1) between chickens and mice
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DOI：
10.1016/j.bbrc.2019.05.129
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发表时间：
2019-07-23
影响因子：
3.1
通讯作者：
Tabata, Shoji
中科院分区：
文献类型：
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作者：
Liang, Ruojun;Kawabata, Yuko;Tabata, Shoji
鸡是重要的工业动物之一，是一种生物学模型动物。本研究以瞬时受体电位香草酸1（TRPV 1）为研究对象，比较鸡和小鼠对酸性刺激的痛觉系统。通过使用全细胞膜片钳系统，我们证实了酸性刺激激活鸡TRPV 1（cTRPV 1）和小鼠TRPV 1（mTRPV 1），但cTRPV 1的峰值电流低于mTRPV 1，并且与mTRPV 1相比，用酸性刺激使cTRPV 1脱敏是困难的。由于TRPV 1的钙调素（CaM）结合位点的C端被认为是TRPV 1脱敏的重要结构之一，我们将鸡的CaM结合位点改造为小鼠的CaM结合位点，构建了嵌合型cTRPV 1（cTRPV 1-mCaM）。我们还比较了天然鸡背根神经节（DRG）细胞与小鼠DRG细胞的酸性反应。TRPV 1-mCaM结果表明，突变型cTRPV 1的脱敏作用与mTRPV 1相似，且突变型cTRPV 1的基础活性显著高于mTRPV 1。与小鼠背根神经节细胞不同，用酸性刺激使鸡背根神经节细胞脱敏也很困难。这些结果表明，鸡和小鼠对酸性刺激的疼痛传导系统存在差异，这是由cTRPV 1的C末端CaM结合位点功能障碍引起的。这些结果表明，鸡反复感到弱疼痛的酸性刺激，没有脱敏。(C)2019爱思唯尔公司All rights reserved.
Chickens, one of the most important industrial animals, are a biological animal model. Here we focused on the transient receptor potential vanilloid 1 (TRPV1) to understand the pain system for acidic stimuli in chickens compared with mice. By using a whole-cell patch clamp system, we confirmed that acidic stimuli activate both chicken TRPV1 (cTRPV1) and mouse TRPV1 (mTRPV1), but the peak current of cTRPV1 is lower than that of mTRPV1, and it is difficult to desensitize cTRPV1 with an acidic stimulus compared to mTRPV1. Since the C-terminal of the calmodulin (CaM) binding site in TRPV1 was reported as one of the important structures for TRPV1 desensitization, we made chimeric cTRPV1 in which the CaM binding site of chicken is changed to that of mouse (cTRPV1-mCaM). We also compared the acidic responses of native chicken dorsal root ganglion (DRG) cells with that of mouse DRG cells. The TRPV1-mCaM results showed that the desensitization of mutant cTRPV1 was similar to that of mTRPV1, and that the basal activities of mutant cTRPV1 were significantly higher than those of cTRPV1. It was also difficult to desensitize the chicken DRG cells with an acidic stimulus, unlike the mouse DRG cells. These results suggest that there are differences in the pain transduction systems for acidic stimuli between chickens and mice that are caused by the dysfunction of the C-terminal CaM biding site of cTRPV1. These results imply that chickens repeatedly feel weak pain from an acidic stimulus, without desensitization. (C) 2019 Elsevier Inc. All rights reserved.