AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS.
AN ELECTRON MICROSCOPE STUDY OF ISOLATED MITOCHONDRIA : METHOD AND PRELIMINARY RESULTS.
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分离线粒体的电子显微镜研究：方法和初步结果。
DOI：
10.1084/jem.81.1.51
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发表时间：
1945-01-01
影响因子：
15.3
通讯作者：
Fullam, E F
中科院分区：
文献类型：
--
作者：
Claude, A;Fullam, E F
1.本文对线粒体的电镜形态作了初步研究。2.本研究的对象是从大鼠淋巴肉瘤中获得的线粒体。通过差速离心法将它们与白血病细胞的其他成分分离，从而可以在电子显微镜下直接检查。3.在纯化的形式中，线粒体呈现为球形体，其中大多数直径约为0.6至1.3 µ。4.电子显微镜下线粒体的某些方面表明这些元件被分化的膜包围。在某些情况下，限制膜似乎负责维持线粒体的一般形状，即使大部分线粒体物质已经丢失。5.通过电子显微镜，可以区分某些线粒体体内直径为80至100 μ m的小元素。进一步的工作建议，以确定这些小颗粒是否是线粒体的正常成分，以及它们之间可能存在什么关系，普通微粒体。6.线粒体作为形态单位的性质进行了讨论。目前的证据表明，线粒体构成明确的物理实体，可以在没有细胞质的情况下持续存在。
1. The present paper constitutes a preliminary study of the morphology of mitochondria by means of electron microscopy. 2. The mitochondria that were the subject of this investigation were obtained from a lymphosarcoma of the rat. They were separated from the other components of the leukemic cells by a method of differential centrifugation, and thus made available for direct examination in the electron microscope. 3. In the purified form the mitochondria appeared as spherical bodies, the majority of them varying in size approximately from 0.6 to 1.3 µ in diameter. 4. Certain aspects of mitochondria in the electron microscope suggest that these elements are surrounded by a differentiated membrane. In some cases the limiting membrane seemed to be responsible for maintaining the general shape of the mitochondria, even when most of the mitochondrial substance had been lost. 5. By means of the electron microscope, it is possible to distinguish small elements, 80 to 100 mµ in diameter, within the body of certain mitochondria. Further work is suggested to establish whether these small granules are normal constituents of mitochondria, and what relation may exist between them and ordinary microsomes. 6. The nature of mitochondria as morphological units is discussed. Present evidence indicates that mitochondria constitute definite physical entities which can persist in the absence of the cytoplasm.