Repeated anabolic/androgenic steroid exposure during adolescence alters phosphate-activated glutaminase and glutamate receptor 1 (GluR1) subunit immunoreactivity in hamster brain: correlation with offensive aggression
Repeated anabolic/androgenic steroid exposure during adolescence alters phosphate-activated glutaminase and glutamate receptor 1 (GluR1) subunit immunoreactivity in hamster brain: correlation with offensive aggression
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DOI：
10.1016/j.bbr.2007.02.025
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发表时间：
2007-06-04
影响因子：
2.7
通讯作者：
Melloni, Richard H., Jr.
中科院分区：
文献类型：
--
作者：
Fischer, Shannon G.;Ricci, Lesley A.;Melloni, Richard H., Jr.
雄性叙利亚仓鼠（Mesocricetus auratus）在青春期（P27-P56）接受中等高剂量（5.0 mg/kg/天）合成代谢/雄激素类固醇（AAS）治疗后，表现出高度升级的攻击性攻击行为。目前的研究检查了青少年 AAS 暴露是否影响磷酸盐激活谷氨酰胺酶 (PAG) 的免疫组织化学定位，PAG 是谷氨酸合成中的限速酶，谷氨酸是一种快速作用的神经递质，与各种物种和攻击模型的攻击性调节有关，以及谷氨酸受体 1 亚基 (GluR1)。仓鼠在青春期期间接受 AAS 治疗，使用居民-入侵者范式对进攻性攻击进行评分，然后检查与攻击性控制有关的大脑区域中 PAG 和 GluR1 免疫反应性的变化。与芝麻油处理的对照动物相比，攻击性 AAS 处理的仓鼠在几个显着的攻击区域中显示出 PAG 数量和含有 GluR1 的神经元的面积密度显着增加，尽管表达的差异模式似乎在大脑区域之间没有重叠。总之，这些结果表明特定攻击区域中谷氨酸合成和GluR1受体表达的改变可能与青少年AAS诱导的攻击性攻击有关。 (c) 2007 Elsevier B.V. 保留所有权利。
Male Syrian hamsters (Mesocricetus auratus) treated with moderately high doses (5.0 mg/kg/day) of anabolic/androgenic steroids (AAS) during adolescence (P27-P56) display highly escalated offensive aggression. The current study examined whether adolescent AAS-exposure influenced the inummohistochemical localization of phosphate- activated glutaminase (PAG), the rate-limiting enzyme in the synthesis of glutamate, a fastacting neurotransmitter implicated in the modulation of aggression in various species and models of aggression, as well as glutamate receptor 1 subunit (GluR1). Hamsters were administered AAS during adolescence, scored for offensive aggression using the resident-intruder paradigm, and then examined for changes in PAG and GluR1 immunoreactivity in areas of the brain implicated in aggression control. When compared with sesame oil-treated control animals, aggressive AAS-treated hamsters displayed a significant increase in the number of PAG- and area density of GluR1-containing neurons in several notable aggression regions, although the differential pattern of expression did not appear to overlap across brain regions. Together, these results suggest that altered glutamate synthesis and GluRl receptor expression in specific aggression areas may be involved in adolescent AAS-induced offensive aggression. (c) 2007 Elsevier B.V. All rights reserved.